Fe_3O_4-SiO_2载体制备和脂肪酶固定化条件优化  

Preparation for Fe_3O_4- SiO_2 carrier and optimization of the conditions for lipase immobilized

作　　者：易笑生 胡铁[2] 冯超[1] 谷政伟 黎继烈[1] 李昌珠[3] 

机构地区：[1]经济林培育与保护省部共建教育部重点实验室(中南林业科技大学),湖南长沙410004 [2]广州航海学院,广东广州510725 [3]湖南省林业科学院,湖南长沙410004

出　　处：《食品工业科技》2015年第1期235-238,243,共5页Science and Technology of Food Industry

基　　金：国家科技支撑计划项目(2011BAD22B04)

摘　　要：通过共沉淀法和溶胶-凝胶法制备了Fe3O4-Si O2磁性纳米粒子,将Fe3O4-Si O2磁性纳米粒子表面进行氨基化修饰得到磁性纳米复合载体,用扫描电子显微镜和傅里叶变换红外光谱对载体进行了表征。通过考察加酶量、戊二醛浓度,固定化时间和温度等因素对蛋白固载率和脂肪酶活力的影响,获得了脂肪酶固定化的最适条件。在0.02g/m L脂肪酶液加入量为8.30m L,戊二醛浓度为8.20%时,在温度24℃条件下,固定化4h,制备的固定化脂肪酶酶活3449U/g。Through co- precipitation and sol- gel method, the Fe3 O4- SiO2 nanoparticles was prepared.The surface of Fe3 O4- SiO2magnetic nanoparticles was modified with amino.Finally, the magnetic nano- composite carrier was obtained.The surface features of the carrier by Scanning electron microscope and Fourier Transform Infrared Spectrometer was described. Through the investigation of the effect of amount of enzyme, concentration of glutaraldehyde,immobilization time and temperature on protein solid load rate and lipase activity,the optimum conditions of lipase immobilization was obtained.Under the condition of the amount of lipase solution （0.02g/mL） was 8.30mL,the concentration of glutaraldehyde was 8.20% ,the temperature was 24~C and the immobilization time was 4h,the obtained immobilized lipase enzyme activity was 3449U/g.

关 键 词：磁性纳米复合载体 脂肪酶 固定化 

分 类 号：TS201.1[轻工技术与工程—食品科学]