有机酸催化水解纤维低聚糖的研究  

Hydrolysis of cellooligosaccharides catalyzed by organic acid

作　　者：马淑玲[1] 彭红[2] 

机构地区：[1]郑州职业技术学院生物工程系,河南郑州450121 [2]南昌大学生物质转化教育部工程研究中心,江西南昌430037

出　　处：《粮食与油脂》2014年第12期24-27,共4页Cereals & Oils

基　　金：国家自然科学基金项目(21306076);江西省教育厅科学技术研究项目(GJJ13089);中国博士后科学基金第54批面上资助项目(2013M540537);江西省博士后科研择优资助项目(2013KY41)

摘　　要：有机酸催化水解纤维低聚糖的能力强弱顺序为:草酸>顺丁烯二酸>丙二酸>乙酸>甲酸>丁二酸,丁二酸不能催化水解纤维低聚糖。二元羧酸水解纤维低聚糖时的能力与其水溶液酸性强弱有关,酸性越强,催化水解能力越大,低聚糖的水解率高。有机酸水解纤维低聚糖是分步进行的,先生成葡萄糖和低一级的低聚糖,低一级的低聚糖再水解生成葡萄糖和更低一级的低聚糖。在初始低聚糖浓度为1.5 mg/m L、水解时间9 h和水解温度95℃的条件下,0.05 mmol/m L草酸分别催化水解纤维三糖、纤维四糖和纤维五糖后产物葡萄糖浓度分别为0.51、0.53和0.13 mg/m L。The order of catalytic activity of organic acids for hydrolysis of cellooligosaccharides is oxalic acid〉maleic acid〉malonic acid〉acetic acid〉formic acid〉butanedioic acid. Butanedioic acid could not catalyze the hydrolysis of cellooligosaccharides. The catalytic activity of biatomic acids is relative to their acidity. The acid with higher acidity resulted in higher conversion of cellooligosaccharides. The hydrolysis of cellooligosaccharides catalyzed by organic acid processed step by step,glucose and cellooligosaccharides with lower degree of polymerization produced first,and the produced oligosaccharides was hydrolyzed further. When cellotriose,cellotetraose,and cellopentaose were hydrolyzed under the conditions of substrate concentration 1.5 mg/mL,oxalic acid dosage 0.05 mmol/mL,reaction time 9 h,and temperature 95℃,the product concentrations of glucose were 0.51, 0.53,and 0.13 mg/mL respectively.

关 键 词：纤维低聚糖 葡萄糖 有机酸 草酸 

分 类 号：TS245.9[轻工技术与工程—制糖工程]