超声波辅助提取三华李花青素及其抗氧化活性研究  

Anthocyanins extraction from Sanhua plums by ultrasonic-assisted and study on its antioxidant ability

作　　者：孔美兰[1] 刘谋泉[1] 陈德宾 

机构地区：[1]韩山师范学院生物学系,广东潮州521041 [2]广东富味制果厂有限公司,广东汕头515064

出　　处：《广东农业科学》2014年第21期92-96,共5页Guangdong Agricultural Sciences

基　　金：广东省重大科技专项(2010A080403004);广东顺大食品调料有限公司委托项目(韩合[2012]161号);广东省教育部产学研结合项目(2009B090300173)

摘　　要：以三华李为原料研究三华李花青素的提取工艺及其抗氧化活性。首先将三华李切成厚度为2 cm的薄片,然后采用单因素和正交试验确定了三华李切片花青素最佳提取工艺参数:以p H值2的70%乙醇溶液作为提取剂,料液比1∶12(W/V),超声波温度55℃,超声波时间50 min,超声波功率400 W。在此条件下,提取液的花青素含量最高,达到21.98μg/L。提取完花青素的三华李切片组织结构完整,可以作为三华李凉果的原料。三华李花青素提取液经AB-8大孔吸附树脂纯化并进行冻干处理,得到三华李花青素粗提物。花青素粗提物含量为2.4 mg/m L时,对羟自由基清除率为51.21%;花青素粗提物含量为0.4 mg/m L时,对超氧阴离子清除率达到48.70%。Extraction technology and antioxidant ability of anthocyanins from no crushing Sanhua plums were investigated. Firstly, mature plums as the raw materials were cut into certain thickness of sheet about 2 cm. Then using single factor and orthogonal method, the optimum extraction conditions were as following： pH 2, ethanol solution （70%） as solvent, I：12（W/V） of material-to-liquid ratio for 50 mix with ultrasonic power 400 W at 55~（2. Under these conditions, the concentration of anthocyanins was up to the maximum, 21.98 I^g/L. The organization of Sanhua plums after extraction was complete and could be used as material of preserved fruit. After purified by AB-8 macro porous resins and freeze-dried, the crude extract of anthocyanidins was obtained. The crude extract of anthocyanidins had good scavenging activity on hydroxyl radical and superoxide anion with scavenging ratio 51.21% of 2.4 mg/mL, and 48.70% of 0.4 mg/mL, respectively.

关 键 词：三华李 超声波辅助提取 花青素 抗氧化性 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]