野生水芹总黄酮提取工艺优化及抗氧化活性  

Optimization Extraction Conditions of Apigenin of Oenanthe javanica(Bl.)DC.and Antioxidant Activity

作　　者：何文兵[1,2] 夏光辉[1,2] 刘欢[1,2] 秦佳梅[3] 

机构地区：[1]通化师范学院制药与食品科学学院,吉林通化134002 [2]通化师范学院长白山食品工程研究中心,吉林通化134002 [3]通化师范学院生命科学学院,吉林通化134002

出　　处：《北方园艺》2015年第1期122-127,共6页Northern Horticulture

基　　金：吉林省科技发展计划资助项目(2013419008CB);吉林省科技发展计划资助项目(20110268);吉林省教育厅"十二五"科学技术研究资助项目(吉教科合字[2013]第495号);通化师范学院校级资助项目(201270)

摘　　要：以长白山区野生水芹为试材,在单因素试验的基础上,通过响应面法优化超声波提取野生水芹总黄酮工艺,并对其抗氧化活性进行考察。结果表明:最佳工艺条件为提取温度70℃、提取功率60W、提取时间9.19min,在此条件下,水芹总黄酮提取率为2.9668mg/g,验证试验得出水芹总黄酮提取率为2.9650mg/g。水芹总黄酮对羟自由基具有较强的清除能力,能很好抑制油脂氧化。利用响应面分析法可优化水芹总黄酮提取工艺,得到理论最佳提取条件,验证结果表明该方法科学、高效。Taking Oenanthe javanica （BI.） growing in Changbai mountain as material,based on single factor experiments, response surface methodology was used to optimize the ultrasonic-assisted extraction flavonoids,and antioxidant activity of total flavonoids were studied. The results showed that,the optimal extraction conditions were extraction temperature 70℃ ;extraction power 60 W; extraction time 9. 19 minutes. Under these conditions, the predicted and experimental extraction yield of apigenin was 2. 9668 mg/g and 2. 9650 mg/g. Extracted total flavonoids had strong scavenging capacity on hydroxyl free base radicals and could better control oil oxidant. In conclusion, response surface method could provide a reliable approach to find optimal conditions for the extraction apigenin from Oenanthe jawanica. The results suggested that the new method was effective and economical.

关 键 词：野生水芹 总黄酮 提取工艺 优化 抗氧化活性 

分 类 号：S636.9[农业科学—蔬菜学]