花青素缓释材料—氨基壳聚糖的制备及缓释性能研究  

Preparation and Release Properties of Amino-Chitosan for Use in Sustained Release of Anthocyanin

作　　者：袁博[1] 许培源[1] 王佩佩[1] 张华[1] 曹成亮[1] 蒋继宏[1] 

机构地区：[1]江苏师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《食品科学》2014年第10期62-67,共6页Food Science

基　　金：江苏省高校自然科学研究项目(13KJD350001);江苏师范大学校自然科研基金项目(12XLB04);江苏省药用植物生物技术重点实验室开放课题(KLBMP1401);江苏省高校优秀科技创新团队资助项目(药用微生物前期开发);徐州市高技术研究重点实验室建设项目(XF12C045)

摘　　要：以壳聚糖为材料,采用反相悬浮交联法制备复合材料,利用Schiff base反应对壳聚糖的氨基进行了保护。利用乙二胺对得到的复合材料进行修饰,获得氨基化的壳聚糖材料,并对其进行结构表征。通过聚乙烯硫酸钾标定氨基,考察环氧丙烷用量对材料的表面游离氨基的影响。分别探讨了致孔剂、预交连剂与交联剂的含量对花青素包封率的影响以及在模拟生理环境中评价材料的缓释情况。结果表明:随着环氧丙烷的含量增加,材料出现游离的氨基减少的情况;随着致孔剂,预交连剂和交联剂的含量增加,花青素的包封率也逐渐增高,但当三者超过一定的含量时,包封率反而出现下降趋势。通过模拟人体生理环境下的花青素的缓释研究,载药颗粒在pH 1.72,温度37℃条件下,4 h内释放量达到了(81.63±1.92)%,在10 h内释放完毕,而在pH 8.25,温度37℃时4 h内释放量达到了(71.68±2.55)%,10 h内基本释放完毕。释放曲线符合正态分布模型,表明该化学修饰的壳聚糖可以作为负载材料用于花青素的缓释用途。Chitosan-based composite materials were prepared using the inverse-phase suspension cross-linking technique by protecting the amino group in chitosan through the Sehiff base reaction. Amino-chitosan was obtained after modification of the composite materials with ethidene diamine and its structure was characterized. In addition, the effect of epoxypropane concentration on free amino groups on the surface of amino-chitosan was investigated; the amount of free amino groups was measured using potassium polyvinylsulfate （PVSK）. Also, the effects of porogen, pre-cross-linking agent and cross-linking agent on the entrapment efficiency of anthocyanin were evaluated as well as sustained release properties of anthocyaninloaded amino-chitosan. The results showed that the amount of free amino group was reduced with increasing epoxypropane concentration. With increasing concentrations of porogen, pre-cross-linking agent and cross-linking agent within certain ranges, the entrapment efficiency of anthocyanin was also gradually increased, but decreased when they exceeded the ranges. Under simulated human physiological conditions, the release of anthocyanin from chitosan particles at pH 1.72 and 37℃ was （81.63 ± 1.92）% in 4 h, and complete release was achieved in 10 h. By contrast, the release of anthocyanin at pH 8.25 and 37℃ was （71.68±2.55）% in 4 h, and the entrapped anthocyanin was almost completely released in 10 h. The release curves, observed to follow normal distribution patterns, showed that the chemically modified ehitosan can be used for sustained release of anthocyanin.

关 键 词：花青素 缓释 氨基壳聚糖 制备 

分 类 号：TS201.2[轻工技术与工程—食品科学]