氢化物发生-原子荧光光谱法测定富硒杂粮中的有机硒和无机硒  

Determination of Organic Selenium and Inorganic Selenium in Selenium-Enriched Grains by Hydride Generation-Atomic Fluorescence Spectrometry

作　　者：刘恒[1] 马盼[1] 王浩东[1] 黄婷[1] 

机构地区：[1]安康学院化学化工系,陕西安康725000

出　　处：《食品科学》2014年第10期170-173,共4页Food Science

基　　金：陕西省科技厅农业攻关项目(2010k01-19);陕西省教育厅科学研究计划项目(12JK0477);安康学院大学生科技创新训练项目(2013aku023);安康学院大学生科技创新训练项目(2013aku022)

摘　　要：采用氢化物-原子荧光光谱法与无水乙醇提取相结合的方法测定了富硒杂粮中总硒、无机硒和有机硒的含量,并对仪器的试验参数进行优化。结果表明,富硒杂粮样品中硒主要以有机硒的形态存在,占硒总量的63.02%-95.10%,其中黄玉米有机硒含量最高,达到95.10%。该方法检出限为0.01 μg/L,总硒回收率为97.31%-103.53%,无机硒回收率96.10%-105.45%。本方法操作简单、灵敏度高、回收率好,适合富硒杂粮有机硒和无机硒的测定。A novel method for the determination of inorganic, organic and total selenium in Se-enriched grains was developed using hydride generation-atomic fluorescence spectrometry （HG-AFS） after anhydrous ethanol extraction. The instrumental parameters were optimized. The results showed that selenium was found to exist mainly in the form of organic Se compounds in selenium-enriched grains in the range of 63.02%-95.10% relative to total Se amount. Among the selenium-enriched grains investigated, yellow corn was the richest in organic Se, accounting for 95.10% of total Se amount. The detection limit （3σ） of the proposed method was 0.01μg/L. Recoveries ranged from 97.31% to 103.53% for total Se and 96.10% to 105.45% for inorganic Se. This method has the advantages of simple operation, high sensitivity and high recovery rate, and is suitable for the determination of organic selenium and inorganic selenium in selenium-enriched grains.

关 键 词：氢化物-原子荧光光谱法 有机硒 无机硒 富硒杂粮 

分 类 号：O657.3[理学—分析化学]