月桂酰氯修饰羊血超氧化物歧化酶工艺优化及酶学性质  

Optimized Preparation and Enzymatic Characterization of Lauroyl Chloride Modified SOD from Sheep Blood

作　　者：杨学山[1] 王本启[1] 王正娟[1] 杨佐青 李远玲[1] 康延良[1] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《食品科学》2014年第7期128-131,共4页Food Science

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2010-03);甘肃农业大学大学生科研训练计划重点项目(20120803)

摘　　要：以羊血为材料获得超氧化物歧化酶(superoxide dismutase,SOD)酶液,采用月桂酰氯修饰SOD,在修饰温度、修饰时间、月桂酰氯添加量和丙酮添加量的单因素试验基础上,通过正交试验优化工艺参数,并比较修饰酶与天然酶的酶学性质。结果表明:10 mL酶比活力为4 348.6 U/mg的SOD样液在修饰温度60℃、修饰时间30 min、月桂酰氯用量0.1 mL、丙酮添加量为酶液体积的1.5倍条件下充分反应,所得修饰酶活力最强;修饰酶热稳定性、pH值耐受性、半衰期等均优于天然酶。月桂酰氯可用于修饰性能较优的羊血超氧化物歧化酶。The process condition for modifying SOD from sheep blood by lauroyl chloride was explored.Four experimental parameters including reaction temperature,reaction time and the amounts of lauroyl chloride and acetone added to SOD solution were investigated by one-factor-at-a-time experiments and optimized by orthogonal array design.The highest enzyme activity was observed for SOD with a specific activity of 4 348.6 U/mg in a 10 mL aqueous solution when modified by reaction with 0.1 mL of lauroyl chloride and 15 mL of acetone for 30 minutes at 60 ℃.The modified SOD exhibited improved heat stability,pH tolerance and half-life compared to the intact one.Therefore,lauroyl chloride can be used to modify sheep blood SOD for better enzymatic performance.

关 键 词：羊血SOD 月桂酰氯 修饰 酶活力 

分 类 号：TS251.9[轻工技术与工程—农产品加工及贮藏工程]