高分子微球免疫吸附剂的制备及其对黄曲霉毒素M_1的吸附性能  

Preparation of Solid Polymer Microsphere Immune Adsorbent and Its Adsorption Performance for Aflatoxin M_1

作　　者：张小舟[1] 甄玉萍 高建伟[2] 王岩[2] 裴世春[2] 

机构地区：[1]齐齐哈尔大学材料科学与工程学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006

出　　处：《食品科学》2014年第8期304-308,共5页Food Science

基　　金：黑龙江省科技厅自然科学基金项目(C201225)

摘　　要：制备用于牛乳中吸附黄曲霉毒M1的免疫吸附剂.以乙醇和水溶液为反应介质,聚乙烯吡咯烷酮为分散剂,偶氮二异丁腈为引发剂,采用分散聚合法制备苯乙烯-甲基丙烯酸缩水甘油酯共聚物微球.利用微球表面的环氧基团将抗黄曲霉毒M1的单克隆抗体固定在微球表面,获得免疫吸附剂.制备的微球粒径约为1.7 μm,微球中环氧基量在67.2％～71.6％之间,制备的免疫吸附剂对乳中黄曲霉毒素M1的吸附量达1.2 μg/g以上.该免疫吸附剂对乳中黄曲霉毒素M1的选择性吸附较好,是去除乳中黄曲霉毒素M1较理想的免疫吸附材料.In this paper,immune adsorbents were prepared for the adsorption of aflatoxin M1 in milk.Copolymer microspheres of glyceryl methacrylate （GMA） and methyl methacrylate （MMA） were prepared using dispersion polymerization with azodiisobutyronitrile （AIBN） as initiator in aqueous ethanol medium in the presence of polyvinylpyrrolidone （PVP） as dispersant under various conditions.Anti-AFM1 monoclonal antibody was coupled with the epoxy groups on the microsphere surface to form an immune adsorbent.The microsphere particle size was approximately 1.7 μm,and the quantity of epoxy group in the microspheres was between 67.2％ and 71.6％.The adsorption quantity of monoclonal antibodies for aflatoxin M1 in milk was more than 1.2 μg/g.These results show that the selective adsorption for aflatoxin M1 in milk of the immune adsorbent is good.It can be an ideal immune adsorption material for the removal of aflatoxin M1 in milk.

关 键 词：共聚物微球 黄曲霉毒素M1 免疫吸附剂 吸附 

分 类 号：TS252[轻工技术与工程—农产品加工及贮藏工程]