超声波提取杨梅色素及其清除超氧阴离子作用  

Ultrasound Pigment Extraction from Waxberry and Ability to Scavenge Superoxide Radicals

作　　者：孔美兰[1] 刘谋泉[1] 张福平[1] 陈德宾 

机构地区：[1]韩山师范学院生物学系,潮州521041 [2]广东富味制果厂有限公司,汕头515064

出　　处：《食品工业》2015年第1期58-62,共5页The Food Industry

基　　金：广东省重大科技专项项目(2010A080403004);广东顺大食品调料有限公司委托项目(韩合【2012】161号);广东省教育部产学研结合项目(2009B090300173)

摘　　要：以杨梅全果为原料,通过单因素和正交试验,进行超声波辅助乙醇提取杨梅色素的工艺研究。结果表明:采用酸醇比V(0.1 mol/L HCl)︰V(95%乙醇)=6︰4的提取剂,在料液比5︰1(m L/g)条件下,采用超声功率180 W,超声温度45℃,超声作用15 min后,再进行50℃恒温水浴震荡浸提30 min,色素提取液在特征波长λ=510 nm处吸光度最大。杨梅色素经过纯化冻干处理后,花色苷含量达到21.51%。杨梅色素具有较强超氧阴离子清除能力,并与质量浓度呈一定正相关关系。Using full waxberry as raw materials, extraction technology of ethanol-soluble pigment assisted by ultrasound was investigated. Through one-factor-at-a-time method and orthogonal method, the optimum extraction conditions were obtained. The conditions of ultrasound pretreatment were as following： component of extract V（0. l mol/L HCI） ： V（95% alcohol）=6 ： 4, 5 ： 1 （mL/g） of material-to-liquid ratio, ultrasound power 180 W, ultrasound temperature 45 ℃, ultrasound time 15 min. After ultrasound pretreatment, pigment extract was extracted at 45 ℃ for 30 rain in shaking bath. The absorbance of extract reached a maximum at characteristic wavelength 510 urn. Through purification and freeze drying, anthocyanin level of pigment was 21.51%. It had a strong activity of scavenging superoxide radicals and had a positive correlation to mass concentration.

关 键 词：杨梅 色素 超声波 花色苷 超氧阴离子 

分 类 号：TS264.4[轻工技术与工程—发酵工程]