鲍鱼脏器胶原蛋白的制备  

Preperation of Ablone Visceral Collagen

作　　者：林丽航[1] 朱芳骞 彭会娟[1] 陈锦权[1] 方婷[1] 

机构地区：[1]福建农林大学食品科学学院,福州350002

出　　处：《食品工业》2015年第1期115-120,共6页The Food Industry

基　　金：福建省重大专项专题项目(编号:kh212001)

摘　　要：以鲍鱼内脏结缔组织为原料,利用酸法提取法和酶法提取法从鲍鱼脏器中提取胶原蛋白。试验先通过单因素试验确定除杂蛋白的试剂,影响效果的因素及水平,并进行正交试验,得到最佳试验方案。用Na OH除鲍鱼内脏结缔组织的杂蛋白的效果最好,并确定除杂蛋白工艺的最佳方案为:浸泡时间为6 h、料液比为1︰8(g/m L)、Na OH浓度为0.10 mol/L;通过单因素试验研究了酸法提取胶原蛋白及酶法提取胶原蛋白的工艺。试验结果表明,酸法提取法提取酸溶性胶原蛋白的最佳方案:醋酸浓度为0.5 mol/L、料液比为1︰10(g/m L)、提取时间为48 h,酶法提取酶溶性胶原蛋白的最佳方案:p H为2.5、加酶量为0.5%、料液比为1︰8(g/m L)、提取时间为48 h。Using abalone visoeml connective tissue as raw material, collagen was extracted by acid extraction method and enzymatic extraction method respectively. The reagent and its levels for removing the non-collagenous protein were investigated through single factor experiments. Then the best experiment parameters were obtained by the orthogonal experiment. NaOH was identified as the best reagent to remove non-collagenous protein. The optimum condition was determined as follows： immersion time 6 h, proportion 1 ： 8 （g/mL） and NaOH conoentration 0.10 mol/L. The single factor experiments of the collagen extraction by acid and enzyme were studied respectively. The results showed that the best process of collagen extraction by acid was detemained as follows： acetic acid concentration 0.5 mol/L, proportion 1 ： 10 （g/mL）, and time 48 h and the best process of collagen extraction by enzyme was determined as follows： pH 2.5, enzyme quantity 0.5%, proportion 1 ： 8 （g/mL） and time 48 h.

关 键 词：鲍鱼内脏 结缔组织 提取 胶原蛋白 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]