胶束电动毛细管色谱分析测定牛奶中的异油酸  

Determination of vaccenic acid in milk by micellar electrokinetic chromatography

作　　者：殷婉露 刘萍[2] 李海星[1] 曹郁生[1] 刘晓华[1] 

机构地区：[1]南昌大学中德联合研究院、食品科学与技术国家重点实验室,江西南昌330047 [2]扬州市第一人民医院,江苏扬州225009

出　　处：《南昌大学学报（理科版）》2014年第5期467-471,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(31260373);江西省科技厅科技计划基金资助项目(20122BBE51007)

摘　　要：为分析测定牛奶中异油酸（t11-C18：1）的含量，建立了毛细管电泳分析异油酸的方法。优化后的电泳条件为：缓冲液含4％（w／v）β-CD、54 mmol·L^-1 SDS、80 mmol·L^-1硼酸盐（pH 9．0）、8 mol·L^-1尿素和4％（v／v）甲醇，电泳电压为25 kV、毛细管柱温为15℃。该方法测定异油酸在100～2500μg·mL^-1范围内的线性关系良好（R2＝0．9817），检出限（LOD）为10μg·mL^-1，平均加标回收率为95．6％。通过该方法测得市售牛奶中异油酸的含量为11．25 mg·g-1乳脂。该方法不仅可以准确测定牛奶中的异油酸，而且可以同时检测牛奶中的主要共轭亚油酸异构体。To determine the content of vaccenic acid （t1 1-C1 8：1 ）in milk,a micellar electrokinetic chromatography method was established in this study.The optimized conditions for buffer solution were 4% （w/v）β-CD,54 mmol·L^-1 SDS,80 mmol·L^-1 borate （pH 9.0）,8 mol·L^-1 urea and 4% （v/v）methanol. The separation voltage was set at 25 kV,and the column temperature was set at 15 ℃.It was found that the calibration curve was linear between the peak area and the concentration in the range of 100-2 500μg ·mL^-1 for vaccenic acid with the correlation coefficient was 0.981 7.The detection limit and the average recovery were 10μg·mL^-1 and 95.6%,respectively.The content of vaccenic acid was 11.25 mg·g-1 fatty acids in milk as detected by micellar electrokinetic chromatography.Moreover,this method ccan ould be used to detect the main conj ugated linoleic acids in milk at the same time.

关 键 词：异油酸 胶束电动毛细管色谱 牛奶 分析 

分 类 号：TS201.2[轻工技术与工程—食品科学]