Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells  

作　　者：Qing Li Jiang Lu Xianyu Wang 

机构地区：[1]Department of Anesthesiology,Taihe Hospital,Hubei University of Medicine [2]Anesthesiology Research Institude,Taihe Hospital,Hubei University of Medicine

出　　处：《Neural Regeneration Research》2014年第22期2002-2007,共6页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Foundation of Hubei Province of China,No.2012FFC060;the Natural Science Foundation of Hubei University of Medicine in China,No.2011QDZR-2;the Provincial Key Disciplines Foundation of Hubei Province of China,No.2014XKJSSJ04

摘　　要：Propofol and remifentanil alter intracellular Ca^2＋ concentration （[Ca^2＋]i） in neural stem/progen-itor cells by activating γ-aminobutyric acid type A receptors and by reducing testosterone levels. However, whether this process affects neural stem/progenitor cell proliferation and differenti-ation remains unknown. In the present study, we applied propofol and remifentanil, alone or in combination, at low, moderate or high concentrations （1, 2–2.5 and 4–5 times the clinically effective blood drug concentration）, to neural stem/progenitor cells from the hippocampi of newborn rat pups. Low concentrations of propofol, remifentanil or both had no noticeable effect on cell proliferation or differentiation; however, moderate and high concentrations of propofol and/or remifentanil markedly suppressed neural stem/progenitor cell proliferation and differen-tiation, and induced a decrease in [Ca^2＋]i during the initial stage of neural stem/progenitor cell differentiation. We therefore propose that propofol and remifentanil interfere with the prolifer-ation and differentiation of neural stem/progenitor cells by altering [Ca^2＋]i. Our ifndings suggest that propofol and/or remifentanil should be used with caution in pediatric anesthesia.Propofol and remifentanil alter intracellular Ca^2＋ concentration （[Ca^2＋]i） in neural stem/progen-itor cells by activating γ-aminobutyric acid type A receptors and by reducing testosterone levels. However, whether this process affects neural stem/progenitor cell proliferation and differenti-ation remains unknown. In the present study, we applied propofol and remifentanil, alone or in combination, at low, moderate or high concentrations （1, 2–2.5 and 4–5 times the clinically effective blood drug concentration）, to neural stem/progenitor cells from the hippocampi of newborn rat pups. Low concentrations of propofol, remifentanil or both had no noticeable effect on cell proliferation or differentiation; however, moderate and high concentrations of propofol and/or remifentanil markedly suppressed neural stem/progenitor cell proliferation and differen-tiation, and induced a decrease in [Ca^2＋]i during the initial stage of neural stem/progenitor cell differentiation. We therefore propose that propofol and remifentanil interfere with the prolifer-ation and differentiation of neural stem/progenitor cells by altering [Ca^2＋]i. Our ifndings suggest that propofol and/or remifentanil should be used with caution in pediatric anesthesia.

关 键 词：nerve regeneration PROPOFOL REMIFENTANIL neural stem cells neural progenitor cells PROLIFERATION apoptosis DIFFERENTIATION [Ca^2＋]i neural regeneration 

分 类 号：R614[医药卫生—麻醉学]