免疫亲和柱净化—高效液相色谱法同时检测小麦中脱氧雪腐镰刀菌烯醇及其衍生物  

Simultaneous determination of deoxynivalenol and ramification in wheat by immune affinity column and high performance liquid chromatography

作　　者：王培 徐广超[2] 尹丽梅 黎睿[4] 谢刚[4] 

机构地区：[1]山东省粮油检测中心,山东济南253303 [2]国家粮食局标准质量中心,北京100037 [3]北京华安麦科生物技术有限公司,北京102002 [4]国家粮食局科学研究院,北京100037

出　　处：《粮油食品科技》2015年第1期63-66,共4页Science and Technology of Cereals,Oils and Foods

基　　金：十二五国家科技支撑计划(2012AA101609)

摘　　要：建立脱氧雪腐镰刀菌烯醇（DON）及其衍生物3-乙酰基—脱氧雪腐镰刀菌烯醇（3-ADON）和15-乙酰基—脱氧雪腐镰刀菌烯醇（15-A-DON）的免疫亲和柱净化—高效液相色谱同时检测方法。小麦样品经超纯水提取、免疫亲和柱净化、吹干、定容后,用配有紫外检测器的高效液相色谱仪进行DON、3-A-DON和15-A-DON三种毒素的同时检测,并与液相色谱质谱法比对。结果表明：该方法检出限,DON、3-A-DON和15-A-DON均为100μg/kg,样品加标回收率范围86.6%～96.5%,变异系数小于10%,具有较好的准确性和精密度。该方法简单、快速、灵敏度高、选择性好,适用于小麦中DON、3-A-DON和15-A-DON的同时检测。The method to determine deoxynivalenol （ DON）, 3 - acetyl - deoxynivalenol （ 3 - A - DON） and 15 - acetyl - deoxynivalenol （ 15 - A - DON ） in wheat simultaneously by immune affinity column clean -up and high performance liquid chromatography was established. The wheat samples were extrac- ted by ultrapure water, cleared up with the immuno -affinity column （IAC）. After drying and constant volume, three toxins, DON,3 -A -DON and15 -A -DON, were detected simultaneously by high per- formance liquid chromatograph with UV detector, and compared with that by Liquid chromatography mass spectrometry. The result showed that the detection limit of method was 100 μg/kg for DON, 3 - A - DON and 15 - A - DON respectively. Recovery rates in wheat spiked with DON, 3 - A - DON, and 15 - A - DON, ranged from 86.6% to 96.5%. The variation coefficient was less than 10%. This method was suitable for determination of DON, 3 - A - DON, and 15 - A - DON in wheat, with the advantages of simplicity, rapidness, sensitivity and good selectivity, veracity and precision.

关 键 词：免疫亲和柱 脱氧雪腐镰刀菌烯醇 3-乙酰基—脱氧雪腐镰刀菌烯醇 15-乙酰基—脱氧雪腐镰刀菌烯醇 高效液相色谱 

分 类 号：R155.5[医药卫生—营养与食品卫生学]