纸浆废料生物炼制细菌纤维素的研究  

Production of bacterial cellulose from waste fiber sludge

作　　者：郭香[1] 张硕[1] 唐敬玉[1] 邹小周 唐晓燕[2] 陈琳[1] 洪枫[1] 

机构地区：[1]东华大学化学化工与生物工程学院微生物工程与工业生物技术研究组,上海201620 [2]环境保护部南京环境科学研究所,南京210042

出　　处：《工业微生物》2015年第1期1-6,共6页Industrial Microbiology

基　　金：教育部新世纪优秀人才支持计划项目(NCET-12-0828);上海市科委项目(11230700600;12nm0500600);中央高校基本科研业务费专项资金资助项目

摘　　要：细菌纤维素(bacterial cellulose,BC)是一种由微生物产生的具有纳米结构的纤维素材料。BC生产的培养基成本偏高,限制了其规模化工业生产和商业应用。为开发新的BC生产原料,通过Cellic CTec 2纤维素酶直接水解硫酸盐和亚硫酸盐两种纸浆废料获得可发酵糖,以其成功制备出BC并研究比较了两种酶解液对BC产量和结构的差异。结果表明,硫酸盐纸浆废料获得的BC产量最高,达9.0 g/L,比亚硫酸盐纸浆废料的7.7 g/L高了17%。两种原料制备的BC膜的结晶度分别为61%和66%,比葡萄糖制备的(78%)低。红外光谱分析表明,不同碳源制备的BC膜的成分没有明显差异。Bacterial cellulose （BC） is a nanostructured polymer product of some bacteria, principally acetic acid bacteria. Compared to plant cellulose, BC has a great potential in wide applications due to its unique properties. Because of the high cost of culture medium, large-scale industrial production and commercial application of BC were limited. In this work, two different waste fiber sludges, sulfate fiber sludge （SAFS） and sulfite fiber sludge （SIFS） were easily hydrolyzed by cellulase without prior thermochemical pretreatment and thereafter were used to produce BC successfully. The yield and structure of BC produced from the two different fiber sludges were compared. The results indicated that the yield of BC from SAFS were 9.0 g/L, which was about 17% higher than that of SIFS （7.7 g/L）. The crystallinity of the BC membranes differed due to the culture media. The crystallinity of BC from SAFS hydrolysate was around 61%, while that from SIFS hydrolysate was around 66% , both of them were lower than that （78%） from the glucose-based reference medium. ATR-FTIR analysis showed that there were no significant differences in the chemical compositions of BC membranes obtained from the two waste fiber sludges and glucose-based media.

关 键 词：细菌纤维素 纸浆废料 酶水解 木醋杆菌 

分 类 号：X793[环境科学与工程—环境工程] TQ920.6[轻工技术与工程—发酵工程]