顶空固相微萃取-全二维气相色谱／飞行时间质谱法分析闽南乌龙茶中的挥发性成分及其在分类中的应用  

Analysis of the volatile components in Minnan oolong tea by headspace solid phase microextraction coupled with comprehensive two-dimensional gas chromatography-time of flight mass spectrometry and the application in its variety identification

作　　者：程权[1] 杨方[2] 李捷[2] 卢声宇[2] 蓝锦昌[2] 江锦彬[2] 

机构地区：[1]食品安全分析与检测教育部重点实验室福建省食品安全分析与检测重点实验室福州大学化学系,福建福州350108 [2]福建出入境检验检疫局检验检疫技术中心福建省检验检疫重点实验室,福建福州350001

出　　处：《色谱》2015年第2期174-181,共8页Chinese Journal of Chromatography

基　　金：国家质检总局科研项目(2013IK147);福建省科技重点项目(2012Y6001)

摘　　要：采用顶空固相微萃取(HS-SPME)结合全二维气相色谱/飞行时间质谱(GC×GC-TOF MS)分析了闽南乌龙茶中的挥发性成分。从48份不同等级和产季的乌龙茶(铁观音、黄金桂、本山、毛蟹和梅占)中获得了2 000余种挥发性化合物,经筛选得到51种共有组分,并结合质谱数据库、保留指数与结构谱图等进行了初步鉴定。在此基础上采用主成分分析法(PCA)获得得分投影图,直观给出了不同样品的分类趋势。通过逐步判别获得9种对分类结果有显著影响的组分,并以此为变量通过Fisher判别法(FDA)建立了4个判别函数,对样品的分类准确率达到97.9%。本试验证实了以挥发性成分识别闽南乌龙茶的可行性。A method to analyze the volatile components in Minnan oolong tea was developed based on headspace solid phase microextraction （HS-SPME） coupled with comprehensive two- dimensional gas chromatography-time of flight mass spectrometry （ GC × GC-TOF MS）. Volatile compounds of 48 oolong tea samples with different qualities and production seasons from five varieties （Tieguanyin, Huangjingni, Benshan, Maoxie and Meizhan ） were extracted by HS- SPME and analyzed by GC×GC-TOF MS. More than 2 000 peaks were obtained from each sam- ple, and 51 common compounds were tentatively identified by comparison with the standard mass spectrum databases, retention indices and structure spectra. The projection score of the common compounds obtained from principal component analysis （PCA） had presented a straightforward classification trend for different oolong tea varieties. In addition, 9 compounds which had significant impact on the classification were selected by stepwise discriminate analy sis, and used as variables to establish four discriminated functions by Fisher＇ s discriminate analysis （FDA）. The accuracy for the recognition of 48 samples was 97.9%. The results had demonstrated the feasibility of the method to be used to discriminate the oolong tea varieties.

关 键 词：顶空固相微萃取 全二维气相色谱/飞行时间质谱 主成分分析 FISHER判别分析 挥发性成分 闽南乌龙茶 

分 类 号：O658[理学—分析化学]