奶粉中香兰素、甲基香兰素和乙基香兰素的测定  

Determination of vanillin, methyl vanillin and ethyl vanillin in milk powder

作　　者：李天宝[1] 刘炜[1] 王春利[1] 李文斌 任忠海[3] 

机构地区：[1]江门出入境检验检疫局,广东江门529000 [2]北京迪马科技有限公司,北京100029 [3]广东检验检疫技术中心,广州510623

出　　处：《中国乳品工业》2014年第12期41-43,共3页China Dairy Industry

基　　金：广东省科技计划项目(2011B010400019);国家质检总局科技计划项目(2014IK169)

摘　　要：建立了同时测定奶粉香兰素、甲基香兰素和乙基香兰素的超高效液相色谱法。样品用水溶解后使用Pro Elut PXA固相萃取柱进行样品前处理净化,C18柱分离,超高效液相色谱仪进行色谱分析。结果表明,香兰素、甲基香兰素和乙基香兰素在0.5~20 mg/L范围内线性关系良好;方法的检出限（S/N=3）分别为0.20,0.25,0.25 mg/L;样品的加标回收率为80.3%~102.7%,相对标准偏差为1.85%~4.11%。该方法结果准确可靠,可实现香兰素、甲基香兰素和乙基香兰素的快速同步分析。An ultra performance liquid chromatography（UPLC） method was developed for the simultaneous determination of vanillin, methyl vanillin and ethyl vanillin in milk powder. Samples were dissolved with water, purified with solid phase extraction column, separated with C18 column, and determined with ultra performance liquid chromatography. The results showed that there were good linear relationships between peak areas and concentrations of vanillin, methyl vanillin and ethyl vanillin in the range of 0.5~20 mg/L, with limits of detection of0.20 mg/L、0.25mg/L and 0.25 mg/L, respectively. The mean recoveries at spiked levels of 2.5~10.0 mg/L were in the range of 79.2%~102.7%, with the relative standard deviations（RSDs, n=6） of 1.85%- 4.11%. This method is sensitive and reliable, and could meet the requirements for simultaneous of vanillin, methyl vanillin and ethyl vanillin in milk powder.

关 键 词：固相萃取 超高效液相色谱 奶粉 香兰素 甲基香兰素 乙基香兰素 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.7[轻工技术与工程—食品科学与工程]