三种蒿属植物叶面腺体观察及其挥发性物质的SPME-GC-MS分析  

Observation Glandular Trichemes and Analysis Volatile Substance for Three Kinds of Artemisia Using SPME-GC-MS

作　　者：唐巧玉[1] 付伟[2] 周毅峰[2] 周大寨[1] 

机构地区：[1]生物资源保护与利用湖北省重点实验室(湖北民族学院),湖北恩施445000 [2]湖北民族学院生物科学与技术学院,湖北恩施445000

出　　处：《湖北民族学院学报（自然科学版）》2014年第4期371-374,共4页Journal of Hubei Minzu University(Natural Science Edition)

基　　金：国家民委项目(12HBZ003);湖北民族学院大学生创新项目(2011B002);生物资源保护与利用湖北省重点实验室重点项目(PKLHB1312)

摘　　要：为了解艾蒿、臭蒿和黄花蒿三种蒿属植物挥发性物质的组成,探寻其与叶面腺毛体的关系,通过顶空固相微萃取和气相色谱-质谱联用法对其化学成分进行鉴定分析比较,并利用体式荧光显微镜观察三种蒿属植物叶面腺毛体数量.结果表明:1黄花蒿挥发性物质种类和含量最多,艾蒿挥发性物质种类和含量其次,臭蒿挥发性物质种类和含量最少,三者主要成分均为单萜类化合物与倍半萜类化合物;2三种植物均含有顺-3-己烯酸乙酯、蒈烯、桉树脑、大根香叶烯、β-石竹烯;3艾蒿挥发性物质还含有罗勒烯、2,6-二甲基-1,5,7-辛三烯-3-羟基、樟脑、β-榄香烯、丁香烯、桉叶稀、杜松烯、E-环氧法尼稀,臭蒿挥发性物质还含有羟基脲、β-蒎稀、β-榄香烯、丁香烯,黄花蒿挥发性物质还含有3-己烯-1-醇、2-辛烯-4-醇、5,5-二甲基-1-乙基-1,3-环戊二烯、邻异丙基甲苯、莰烯、D-柠檬稀、β-蒎稀、E-β-法尼烯;4三种植物中黄花蒿挥发性物质种类和含量最多,其腺毛体密度也最大;艾蒿挥发性物质种类和含量其次,其腺毛体密度也处于其次;臭蒿挥发性物质种类和含量最少,其腺毛体密度也最小.In order to know the volatile substances of Artemisia argyi H.Lév.et Vaniot,Artemisia hedinii and Artemisia annua L.and explore its relationship with the number of glandular trichomes on leaf,head-space solid-phase micro extraction and gas chromatography-mass spectrometry are used to analyze and i-dentify the chemical components,and using type fluorescent microscope to observe the number of glandu-lar trichome.The results show that①the species of Artemisia annua L volatile substances is most, the spe-cies of Artemisia argyi H.Lév.et Vaniot volatile substances is second, the species of Artemisia hedinii volatile substances is least.The main constituents are monoterpenes and sesquiterpene;②All contain cis-Ethyl 3-hexenoate,karen, Cineole, Germacrene,β-Caryophyllene;③Artemisia argyi volatile substances also contains Ocimene, 2, 6-Dimethyl-1, 5, 7-octatriene-3-ol, DL-Camphor, β-Elemene, Caryo-phyllene,π-selinene, Cadinene, E-β-Farnesene, Artemisia hedinii volatile substances also contain Hydroxycarbamide,β-pinene,β-Elemene, Caryophyllene, Artemisia annua L.volatile substances also contains 3-Hexen-1-ol,2-Octen-4-ol,1-isopropyl-2-methylbenzene,β-pinene,D-limonene,5,5-dimethyl-1-ethyl-1,3-cyclopentadiene,Camphene, E-β-Farnesene;④The species and contents of vol-atile substances and glandular trichomes of Artemisia annua L.are most;The species and contents of vola-tile substances and glandular trichomes of Artemisia argyi are second;The species and contents of volatile substances and glandular trichomes of Artemisia hedinii.are least.

关 键 词：艾蒿 臭蒿 黄花蒿 腺毛体 挥发性物质 顶空固相微萃取-气质联用 

分 类 号：O657.63[理学—分析化学]