辐照对克氏原螯虾致敏蛋白质生化性质的影响  

Effects of Irradiation on Biochemical Properties of Allergic Protein in Procambarus clarkii

作　　者：廖涛[1] 叶敏[1] 熊光权[1] 李新[1] 江洪有 鉏晓艳[1] 耿胜荣[1] 叶丽秀[1] 夏和舟[1] 程薇[1] 

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所/湖北省农产品辐照工程技术中心,武汉430064

出　　处：《湖北农业科学》2014年第24期6082-6085,共4页Hubei Agricultural Sciences

基　　金：公益性行业(农业)科研专项(201103007);国家科技支撑计划项目(2012BAD28B06);湖北省农业科技创新中心项目(2014-620-007-001)

摘　　要：通过对克氏原螯虾(Procambarus clarkii)致敏蛋白质的提取,利用60Coγ射线对其进行辐照,探讨了7个不同辐照剂量10、20、30、40、50、60、70 k Gy对克氏原螯虾致敏蛋白质降解效果的影响,以及对致敏蛋白质浓度、浊度和疏水性等生化性质的影响。结果表明,随着辐照剂量的增加,致敏蛋白质条带逐渐减弱,部分条带消失,即使是最大辐照剂量70 k Gy也未能使所有致敏蛋白质条带完全降解;与未辐照的克氏原螯虾致敏蛋白质相比,辐照后的蛋白质浓度降低,浊度增高,疏水性逐渐增强,说明辐照对克氏原螯虾致敏蛋白质有一定的降解作用。Allergic protein in procambarus clarkii was extracted. Seven different radiation doses （10, 20, 30, 40, 50, 60, 70 kGy） of ^60Co γ-ray were used to study the effects of irradiation on allergic protein in Procambarus clarkii. The results showed that the protein bands gradually weakened with the increase of radiation dose. The allergenic protein bands were not degraded completely under the maxium radiation dose of 70 kGy. Compared with the unirradiated allergic protein in Procambarus clarkii, concentration of protein decreased. The turbidity and hydrophobicity of protein increased gradually. It is indicated that the irradiation can degrade allergic protein in Procambarus clarkii.

关 键 词：^60Coγ射线 克氏原螯虾(Procambarus clarkii) 辐照降解 致敏蛋白质 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程] R154[轻工技术与工程—食品科学与工程]