长白山核桃楸种仁抗氧化肽制备及其活性研究  

Preparation of Antioxidant Peptide from Juglans mandshurica Maxim Kernel in Changbai Mountain and its Activity

作　　者：孙琳琳[1,2] 闵伟红[1,2] 冷帅辰 李京京[1,2] 刘景圣[1,2] 郑鸿雁[1,2] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]小麦和玉米深加工国家工程实验室,吉林长春130118

出　　处：《食品研究与开发》2014年第24期5-9,共5页Food Research and Development

基　　金：国家"863"计划项目(2013AA102206)

摘　　要：以长白山核桃楸种仁分离蛋白为原料,采用Box-Benhnken响应面法对影响还原能力的主要因素p H、温度、加酶量和底物浓度进行研究。确定其最佳工艺参数为:p H7.6,酶解温度50℃,加酶量4 510 U/g,底物浓度4.6%,时间4 h,该条件下其还原能力为0.35,水解度为16.50%。抗氧化活性试验表明,随着分离蛋白酶解产物浓度的增加,对DPPH、ABTS、·OH的清除作用及还原能力逐渐增加。当酶解产物浓度达到0.8 mg/m L时,对ABTS的清除率为VC的100%,当浓度达到1.2 mg/m L时,对DPPH的清除率达到相同浓度VC的90.08%。浓度继续增加到15 mg/m L时,对·OH的清除作用为VC的94.56%;这说明优化后的核桃楸种仁分离蛋白酶解产物对DPPH和ABTS具有较强的清除作用,对·OH的清除作用比VC弱。With protein isolate from Juglans mandshurica Maxim kernel in Changbai mountain as the raw material,response surface methodology was applied to optimize the hydrolysis conditions （including pH, temperature, enzyme dosage, and substrate concentration） with reducing power ability as index. The optimum extraction conditions are as follows： pH value of 7. 6, substrate concentration of 4. 6 %, enzyme dosage of 4 510 U/g, enzymolysis temperature of 50℃, and time of 4 h. On this condition, the reducing power ability was 0. 35, the degree of hydrolysis was 16. 50%. The results of antioxidant tests showed that scavenging activity on DPPH, ABTS,·OH and reducing power ability increased with increasing enzymatic hydrolysate concentration. The scavenging activity of enzymatic hydrolysate on DPPH and ABTS was 90. 08%and 100%of VC at 1. 2 mg/mL and 0. 8 mg/mL, the scavenging activity on ·OH was 94. 56%of VC at 15 mg/mL. These demonstrated that the scavenging activity of enzymatic hydrolysate on DPPH and ABTS are strong, the scavenging activity on·OH are weaker than those of VC.

关 键 词：长白山核桃楸 分离蛋白 响应面 抗氧化活性 

分 类 号：TS255.6[轻工技术与工程—农产品加工及贮藏工程]