响应面分析法优化雨生红球藻产虾青素培养基  

Optimization of Haematococcus pluvialis medium producing astaxanthin by response surface methodology

作　　者：徐建春 孙翰[2] 张睿钦[2] 管斌[2] 孔青[2] 耿兆艳 李霞 

机构地区：[1]青岛琅琊台集团股份有限公司,山东青岛266400 [2]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国酿造》2014年第12期72-75,共4页China Brewing

基　　金："十二五"农村领域国家科技计划(2013BAD10B02-06);山东省科技计划项目(2014GSF121029);啤酒生物发酵工程国家重点试验室开放课题(K2012002)

摘　　要：通过单因素试验确定雨生红球藻细胞转化产虾青素培养基中乙酸钠、硝酸钾和磷酸二氢钾的较佳质量浓度范围。研究表明,磷酸二氢钾对雨生红球藻产虾青素的影响不大,而乙酸钠和硝酸钾含量对虾青素产量影响较显著。利用响应面法优化,得到乙酸钠1.460 5 g/L,硝酸钾0.008 4 g/L。优化后虾青素产量从0.977 4 mg/L提高到10.908 mg/L,提高了9.16倍。The concentration range of sodium acetate,potassium nitrate and potassium dihydrogen phosphate content in Haematococcus pluvialis medium for astaxanthin production was determined by single factor experiments.The results showed that the concentration of potassium dihydrogen phosphate had little effect on astaxanthin production.While the sodium acetate and potassium nitrate concentration significantly affect astaxanthin yield.Response surface methodology was employed to optimize the two factors for astaxanthin production.The optimal medium composition for astaxanthin production was sodium acetate 1.460 5 g/L and potassium nitrate 0.008 4 g/L.This astaxanthin yield of the optimized medium increased from 0.977 4 mg/L to 10.908 mg/L,which increased 9.16 times.

关 键 词：雨生血球藻 虾青素 培养基优化 响应面法 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]