ELISA试剂盒法与GC—MS法检测红酒中塑化剂的比较研究  

Using GC—MS and ELISA kits for comparative analysis of plasticizers in red wine

作　　者：曹必溥 曹庸[2] 苗建银[2] 曹素芳[2] 刘果[2] 谭荣威 

机构地区：[1]华南农业大学生命科学学院,广东广州510642 [2]华南农业大学食品学院,广东广州510642

出　　处：《食品与机械》2015年第1期82-85,115,共5页Food and Machinery

基　　金：国家科技支撑计划(编号:2012BAK17B00)

摘　　要：采用气相色谱—质谱法和ELISA试剂盒法对比分析红酒中塑化剂含量。通过改进气相色谱—质谱法样品检测前处理条件,以及两种检测方法的对比分析,研究适合红酒中塑化剂的检测方法。结果表明：ELISA试剂盒法与GC—MS法两种方法的前处理对塑化剂的萃取效果相差不大,且检测出红酒中塑化剂的主要种类为DIBP、DBP、DEHP。GC—MS法检测红酒中塑化剂具有高精准度和高回收率,完全满足红酒中塑化剂检测的要求;试剂盒法的检测值比GC—MS法的检测值要大3.4-5.5倍,但是这不影响ELISA试剂盒法作为一种初步的快速检测方法的应用。There were two methods to detect the plasticizers of red wine.The first one was a traditional method（GC-MS）and another one was a new method-ELISA kit.An improved pre-processing of GC-MS and the comparative analysis of the two methods was used to find a novel detection method.The results：there was a little difference for the rate of plasticizer extraction with different pretreatment.And DIBP,DBP,DEHP were detected.The GC-MS method demonstrated high precision and recovery.The GC-MS method was a excellent method to detect the plasticizers of red wine.The detection results of ELISA kit were 3.4to 5.5times larger than the GC-MS method.It was enough for ELISA kit as a rapid detection method of the plasticizers of red wine.

关 键 词：GC—MS ELISA 红酒 塑化剂 

分 类 号：TS261.7[轻工技术与工程—发酵工程] O657.63[轻工技术与工程—食品科学与工程]