复合蛋白酶水解玉米谷蛋白产物的抗氧化活性研究  

Antioxidant activity of hydrolysate from corn glutelin by protamex

作　　者：刘玥[1,2] 刘晓兰[1,2] 郑喜群[1,2] 周利敏[1,2] 

机构地区：[1]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006 [2]农产品加工黑龙江省普通高校重点实验室,黑龙江齐齐哈尔161006

出　　处：《食品与机械》2015年第1期141-145,共5页Food and Machinery

基　　金：国家自然科学基金资助项目(编号:31371726)

摘　　要：以玉米谷蛋白为原料,利用复合蛋白酶对其进行限制水解,探讨不同水解时间所获得的谷蛋白酶解物的分子量分布和抗氧化活性。结果表明：不同水解时间获得的酶解物分子量分布差异较大。水解时间为120min的酶解物中,分子量分布为6 511.51-307.32Da的肽段占94.36%,此时获得的酶解物的抗氧化活性最高,其对DPPH自由基、O-2·、·OH的清除率分别为58.86%,82.64%,37.21%;还原力为0.236;与亚铁离子的螯合能力为29.92%。The molecular weight distribution and antioxidative activities of corn glutelin hydrolysate by Protamex during hydrolysis process,corn glutelin as raw material,were investigated.The results showed that the highest antioxidant activities was exhibited at the hydrolysis time of 120 min,with molecular weight distribution among 6 511.51-307.32 Da and peptides component 94.36%.DPPH radical scavenging activity,superoxide anion scavenging activity,hydroxyl radical scavenging activity,reducing power and Fe2＋-chelating activity of hydrolysate were 58.86%,82.64%,37.21%,0.236 and 29.92%,respectively.

关 键 词：蛋白酶 水解 玉米 谷蛋白 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]