高产D-塔格糖植物乳杆菌WU14的筛选与鉴定  

Study on screening and identification of Lactobacillus plantarum WU14 with high D-tagatose yield

作　　者：应碧 张艳丽[1] 周通[1] 钟平安[1] 罗燕萍[1] 陶然[1] 邱礼城 徐波[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《中国饲料》2015年第4期17-20,共4页China Feed

基　　金：江西省科技支撑计划社会发展领域项目(20142BBG70015);南昌市科技局南昌市党外专家博士产学研合作项目;江西省大学生创新创业训练计划项目(DC201341)

摘　　要：D-塔格糖是一种天然低热量甜味剂。L-阿拉伯糖异构酶(L-AI)是生物法异构化D-半乳糖为D-塔格糖的关键酶。本研究从腌菜和泡菜中分离出一批乳酸菌,经薄层色谱法初筛和改良半胱氨酸咔唑法复筛,获得一株高产D-塔格糖的乳酸菌,其发酵粗酶液中L-阿拉伯糖异构酶酶活达13.95 U/m L,经16S r DNA序列比对及生化特征分析,该菌被鉴定并命名为Lactobacillus plantarum WU14。研究结果可为生物转化D-塔格糖达到工业化生产奠定基础。D-tagatose is a kind of natural sweetener with low-calorie.L-arabinose isomerase is the key enzyme for D-galactose isomerization to D-tagatose by biological method.In this study lactic acid bacteria strains were isolated from pickled vegetables and pickled cabbage.A lactic acid bacteria strain with high yield of D-tagatose was screened by the thin layer chromatography and modified eysteine carbazole method from the lactic acid bacteria strains and its L-arabinose isomerase activity was 13.95 U/mL after analysed.This strain was named Lactobacillus plantarurn WUI4 based on the sequence analysis of 16S rDNA and biochemical characteristics.The result of this study could lay the foundation for the bioconversion D-tagatose industrial production.

关 键 词：D-塔格糖 植物乳杆菌WU14 L-阿拉伯糖异构酶 

分 类 号：S816.7[农业科学—饲料科学]