miRNA-210在胃癌转移细胞株中的表达及功能预测  

Expression of mi RNA-210 in gastric cancer cell lines and its function prediction

作　　者：张小静[1] 冯先玲[1] 黄勇[2] 高燕 曹子一 黄伟玲[2] 张媛[2] 简千贺 钟锦成 杨梦婷[2] 范新民[1] 金哲[1,5] 

机构地区：[1]深圳大学医学院病理教研室,深圳天然小分子创新药物工程实验室,深圳市肿瘤转化医学重点实验室,广东省深圳市518060 [2]深圳大学医学院临床医学系,广东省深圳市518060 [3]深圳市第六人民医院(南山医院)消化科,广东省深圳市518060 [4]深圳大学医学院药学系,广东省深圳市518060 [5]北京大学深圳研究生院化学基因组学重点实验室,广东省深圳市518060

出　　处：《世界华人消化杂志》2015年第1期78-84,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金面上资助项目;No.81172282;国家自然科学基金青年基金资助项目;No.81302151;深圳市海外高层次人才创新创业专项基金资助项目;No.KQCX20130621101141669;深圳市科技研发资金国家和省计划配套基金资助项目;No.GJHS20120621142654087;深圳市肿瘤转化医学重点实验室建设基金资助项目;No.ZDSY20130329101130496;深圳大学科研基金资助面上资助项目;No.80100035905;深圳大学基础研究科研基金资助项目;No.201108;深圳大学科技创新团队基金资助项目;No.T201202~~

摘　　要：目的:筛选胃癌(gastric cancer,GC)转移相关的差异表达微小RNA(micro RNA,miR NA),探讨miR NA-210与GC转移的可能机制.方法:应用mi RNA表达谱芯片筛选GC高转移细胞株RF48及低转移细胞株RF1的差异表达mi RNAs,RT-PCR检测mi RNA-210在7种GC细胞株中的表达情况,利用mi RWalk软件获得mi RNA-210的靶基因,通过David在线软件(包括GO分析及KEGG通路预测)分析mi R-210靶基因的可能生物学功能.结果:与RF1相比,mi RNA芯片在RF48细胞中共筛选出21个表达上调和15个表达下调的mi RNA,其中mi RNA-210在高转移细胞株RF48中的表达增高;RT-PCR结果显示m i R N A-210在高转移G C细胞株中表达增高(P<0.05);mi RWalk共获得155个mi RNA-210靶基因;GO分析及KEGG pathway分析得到miR NA-210靶基因功能,这些靶基因参与了肿瘤的发生、发展及转移.结论:利用mi RNA芯片技术获得了GC转移相关mi RNA表达谱;mi RNA-210的异常表达可能与GC的转移有关,为GC转移的早期诊断及发现新的治疗靶点奠定了基础.AIM： To screen microRNAs （miRNAs） associated with metastasis of gastric cancer （GC） by miRNA microarray and to explore the possible role of miRNA-210 in GC metastasis by bioinformatics. METHODS： GC cell lines with low （RF1） or high metastatic potential （RF48） were used for miRNA expression profiling using human miRNA microarray. Expression of miRNA-210 in 7 GC cell lines was detected by RT-PCR. MiRNA-210 targets were obtained using miRWalk, and functions of these targets in GC were predicted with David online. RESULTS： Compared with RF1 cells, 21 and 15 miRNAs were up-regulated and down- regulated in RF48 cells, respectively. Expression of miRNA-210 was further validated by realtime quantitative RT-PCR in multiple GC cell lines with different metastatic potential, which showed that miRNA-210 was overexpressed in GC cell lines with high metastatic potential. Bioinformatics analysis suggested that miRNA-210 was related with tumorgenesis and metastasis.CONCLUSION： Screening miRNAs associated with metastasis lays a foundation for identifying early diagnostic markers and new therapeutic targets for GC metastasis. Expression profile of miRNAs associated with metastasis was obtained by miRNA microarray; dysregulated expression of miRNA-210 may be related with GC metastasis, and may serve as an early diagnostic biomarker and new treatment target.

关 键 词：胃肿瘤 miRNA-210 微阵列分析 肿瘤转移 计算生物学 

分 类 号：R735.2[医药卫生—肿瘤]