米根霉无载体固定化发酵产L-乳酸  

Production of L-Lactic Acid by Self-immobilized Rhizopus oryzae

作　　者：陈晓佩[1,2] 张丽[1,2] 顾夕梅 李鑫[1,2] 勇强[1,2] 余世袁[1,2] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037 [2]江苏省生物质绿色燃料与化学品重点实验室,江苏南京210037

出　　处：《林产化学与工业》2014年第5期127-132,共6页Chemistry and Industry of Forest Products

基　　金：江苏省自然科学基金项目(BK20131426);国家自然科学基金资助项目(31000278);教育部高等学校博士学科点专项科研基金项目(20103204120009)

摘　　要：通过研究米根霉NLX-M-1利用葡萄糖无载体固定化发酵产L-乳酸的影响因素,获得米根霉利用木质纤维基葡萄糖进行无载体固定化发酵的最优条件。优化的米根霉无载体固定化产L-乳酸条件:初始葡萄糖质量浓度100 g/L,(NH4)2SO4质量浓度2 g/L,接种量2%(体积分数),CaCO330 g/L,KH2PO40.1 g/L,MgSO4·7H2O 0.25 g/L,ZnSO4·7H2O 0.1 g/L。在优化条件下,以纯葡萄糖为碳源的米根霉发酵过程,形成平均直径1 mm的微球,L-乳酸产量为76.6 g/L,转化率为81.6%。以玉米秸秆酸爆渣酶解葡萄糖浓缩至60 g/L进行L-乳酸发酵,米根霉形成直径约1.2 mm的微球,L-乳酸产量为36.4 g/L,转化率为63.5%。The main factors affecting self-immobilization of Rhizopus oryzae NLX-M-1 for L-lactic acid production were studied in present study. The optimum fermentation conditions utilizing wood fiber-based glucose for R. oryzae self-immobilization were obtained. The optimal results were achieved as 100 g /L initial glucose,2 g /L（ NH4）2SO4,2 %inoculum size（ value fraction）,30 g /L CaCO3,0.1 g/L KH2PO4,0.25 g/L MgSO4·7H2O and 0. 1 g /L ZnSO4·7H2O. Under this optimal condition,R. oryzae formed pellets with an average diameter of about 1 mm during the fermentation,the production of L-lactic acid was 76. 6 g /L and the conversion rate was 81. 6 %. R. oryzae in acid explosive residue hydrolyzate containing 60 g /L glucose became 1. 2 mm pellets,and produced 36. 4 g /L L-lactic acid with a conversion rate of 63. 5 %.

关 键 词：米根霉 无载体固定化 L-乳酸 玉米秸秆 酶解葡萄糖 

分 类 号：TQ35[化学工程]