不同补料方式对酿酒酵母高密度发酵的影响  

Effect of fed-batch fermentation modes on high density fermentation of Saccharomyces cerevisiae

作　　者：荣博涵[1] 甄玉国[1,2] 赵小丽[1] 刘乙辰[1] 冀红芹[1] 

机构地区：[1]吉林农业大学吉农博瑞奶牛饲料研发中心,吉林长春130118 [2]长春博瑞饲料集团有限公司技术中心,吉林长春130114

出　　处：《中国酿造》2015年第2期72-75,共4页China Brewing

基　　金：吉林省重大科技攻关项目(2012ZDGG006)

摘　　要：以酿酒酵母为发酵菌种,使用自主优化筛选配制的糖蜜培养基,分别采用残糖反馈分批补料和连续流加补料方式,优化发酵工艺条件以提高发酵液中菌体密度.残糖反馈分批补料,拟设定3个梯度,分别控制对数期发酵液中残糖含量在0.5％～1.5％,1.0％～2.0％,1.5％～2.5％.三次试验结果分别为,试验1菌体培养32 h时达到最大生物量,为28.88 g/L;试验2菌体培养52 h时达到最大生物量,为27.43 g/L;试验3菌体培养24 h时达到最大生物量,为22.06 g/L.通过连续流加补料培养菌体,结果培养30 h时达到最大生物量,为29.29 g/L.Using Saccharomyces cerevisiae as fermentation starter, the fermentation process for improving S. cerevisiae density in the laboratory screened single molasses medium was optimized by residual glucose feedback fed-batch and continuous flow plus feeding mode. The fed-batch of glucose feedback test was set three gradients to control the content of residual glucose （soluble glucose） in the fermentation broth of0.5%-1.5%, 1.0%- 2.0%, 2.0%-2.5%, respectively. The results showed that yeast cells in test 1 fermentation broth （0.5%-1.5%） reached the maximum biomass concen- tration （MBC） with dry cell weight （DCW） of 28.88 g/L at 32 h; yeast cells in test 2 （1.0%-2.0%） fermentation broth reached the MBC with DCW of 27.43 g/L in 52 h; and yeast cells in test 3 （1.5%-2.5%） fermentation broth reached the MBC with DCW of 22.06 g/L in 24 h. The feeding test results with continuous flow showed that yeast cells in the fermentation broth reached the MBC （DCW=29.29 g/L） in 30 h.

关 键 词：糖蜜 高密度发酵 残糖反馈分批补料 流加补料 

分 类 号：Q93-33[生物学—微生物学]