响应面法优化真菌复合系F-7产纤维素酶的发酵培养基  

Optimization of Medium Composition for Cellulase Production by Fungus Community F-7 Using Response Surface Methodology

作　　者：尚婷婷[1] 陈静[1] 李全宏[2] 邓尚贵[1] 

机构地区：[1]浙江海洋学院食品与医药学院,舟山316000 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品工业》2015年第3期162-166,共5页The Food Industry

基　　金：国家重大科技合作项目(2012DFA30600);浙江省科技厅公益技术应用研究项目(2012C22063)

摘　　要：为提高复合菌系降解纤维素的酶活力,通过响应面法对其培养基成分进行优化。首先对其液体发酵产酶培养基进行优化,在此优化基础上,利用Plackett-Burman试验筛选出影响真菌复合菌系F-7产纤维素酶活的显著因素,再采用最陡爬坡路径逼近最大响应区域,结合Box-Behnken响应面法对显著因素进行分析,优化得到最佳发酵培养基。(NH4)2SO4、MgSO4和秸秆为F-7复合菌系产纤维素酶活的显著性因素,优化后获得最佳培养基成分为玉米芯5 g/L,秸秆7.5 g/L,(NH4)2SO4 4 g/L,K2HPO4 1 g/L,MgSO4·7H2O 1.2 g/L,KH2PO4 1 g/L,NaCl 1 g/L,蛋白胨1 g/L,酵母膏1 g/L。结果表明,优化后纤维素酶活由为50.12μmol/m L提高到68.73μmol/m L,提高了41%。The research aimed to improve the cellulose enzyme activity of composite microbial system,response surface analysis was used to optimize the culture medium composition.The culture medium was first optimized through Plackett-Burman experiments to explore the effects of major factors on the cellulase production by fungus community F-7.Steepest ascent procedure and response surface methodology based on Box-Behnken experimental design were employed to optimize the medium components.The results showed that ammonium sulfate,magnesium sulfate and straw were the most significant factors.The optimized medium ratio was as followings：corncob 5 g/L,straw 7.6 g/L,（NH4）2SO4 4 g/L,K2HPO41 g/L,MgSO4·7H2O 1.2 g/L,KH2PO4 1 g/L,NaCl 1 g/L,peptone 1 g/L and yeast extract 1 g/L.Under the optimal condition,the enzyme activity was increased by 41%,from 50.12 μmol/mL to 68.73 μmol/mL.

关 键 词：响应面分析 纤维素酶 发酵 培养基优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]