基于单纯形-重心设计的菜籽粕生物改良菌株的复配研究  

Complex formulation of biologically modified strain for rapeseed meal by simplex-centroid mixture design

作　　者：钮琰星[1,2] 倪光远 万楚筠[1,2] 金虎[1,2] 郭勉[1,2] 黄庆德[1,2] 黄凤洪[1,2] 

机构地区：[1]中国农业科学院油料作物研究所,武汉430062 [2]油料脂质化学与营养湖北省重点实验室,武汉430062 [3]深圳市市场监督管理局,广东深圳518040

出　　处：《中国油脂》2015年第2期72-76,共5页China Oils and Fats

基　　金：国家自然科学基金(31201461);国家科技支撑计划(2010BAD01B07)

摘　　要：硫甙和中性洗涤纤维是限制菜籽粕饲用的两个重要抗营养因子。发酵法是降低菜籽粕抗营养因子的重要手段。通过初筛、复筛和混料设计,优选了混合菌发酵菌株和接种比例。在初筛中采用菜籽粕鉴定培养基,在复筛中对硫甙降解率、中性洗涤纤维降解率、生长曲线和蛋白酶酶活进行综合评价,在复筛的基础上采用单纯形-重心设计进行菌株配比的优化,拟合出因素与响应值之间的回归方程,得到优化的菌株接种比例为枯草芽孢杆菌B3 46%、纳豆芽孢杆菌N1 30%、酿酒酵母R1 24%,在此条件下进行发酵试验,硫甙、中性洗涤纤维的降解率分别为94.63%、24.17%。Glucosinolates and neutral detergent fiber are two important anti- nutritional factors that limit the feeding value of rapeseed meal. Fermentation is a good method to decrease the anti- nutritional factors in rapeseed meal. The mixed strains and inoculation ratio were optimized by prescreening,the second screening and mixing design. Identification medium of rapeseed meal was used in prescreening,then the degradation rates of glucosinolates and neutral detergent fiber,the growth curve and protease activity were comprehensively evaluated during the second screening. The inoculation ratio was optimized by simplex- centroid mixture design on the basis of the second screening,then the regression equation between factor and response value was fitted. The result showed that the optimal inoculation ratio was obtained as follows： Bacillus subtilis B3 46%,Bacillus natto N1 30% and Saccharomyces cerevisiae R1 24%. Under the optimal condition,the degradation rates of glucosinolates and neutral detergent fiber were 94. 63%and 24. 17% respectively.

关 键 词：菜籽粕 固态发酵 菌株 单纯形-重心设计 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] S816[轻工技术与工程—食品科学与工程]