SPME-GC-MS结合保留指数分析黄连木挥发性成分  

SPME-GC-MS Combined with Kovat's Retention Index Analysis for Volatile Components in Pistacia chinensis

作　　者：余倩[1] 宋双红[2] 李翠芹[1] 

机构地区：[1]陕西师范大学药用资源与天然药物化学教育部重点实验室/西北濒危药材资源开发国家工程实验室/生命科学学院,陕西西安710119 [2]西北工业大学生命学院,陕西西安710072

出　　处：《中药材》2014年第11期2022-2025,共4页Journal of Chinese Medicinal Materials

基　　金："十二五"国家科技支撑计划项目(2011BA106B06);中央高校基本科研业务费专项资金资助(GK201303009)

摘　　要：目的:分析比较陕西产黄连木果实和叶中的挥发性成分。方法:采用顶空固相微萃取法(SPME)提取黄连木果实和叶中的挥发性成分,用气相色谱-质谱技术(GC-MS)结合保留指数分析和鉴定化学成分。结果:从黄连木的果实和叶中分别鉴定出29种和17种挥发性成分,分别占总峰面积的95.30%和96.91%。黄连木果实和叶的共有成分13种。结论:陕西产黄连木果实和叶中挥发性成分在组成和含量上存在差异,它们的主要组分是萜类化合物,但黄连木果实以单萜(76.32%)类化合物为主,黄连木叶以倍半萜(65.42%)类化合物为主。Objective： To analyze and compare the volatile components in fruits and leaves of Pistacia chinesis. Methods： The volatile components were extracted from the fruits and leaves of Pistacia chinesis by solid-phrase microextration,and were analyzed and identified by gas chromatography-mass spectrometry（ GC-MS） combined with Kovat＇s retention index. The relative content of each component was calculated by normalization method. Results： 29 and 17 volatile components were identified from the fruits and leaves respectively,representing the relative content of 95. 30% and 96. 91% of the volatile components. 13 common components were identified in both the fruits and leaves. Conclusion： The volatile components in the fruits vary from that in the leaves in type and content,terpenoids are major components in the fruits and leaves of Pistacia chinesis in Shaanxi. Monoterpenes（ 76. 32%） are the major components of the fruits,while sesquiterpenes（ 65. 42%） are the major components of the leaves.

关 键 词：黄连木 固相微萃取 挥发性成分 气相色谱质谱联用 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]