黑曲霉发酵水稻秸秆产纤维素酶的研究  

Study on cellulase production by Aspergillus fermentation of rice straw

作　　者：孙科[1] 

机构地区：[1]徐州生物工程职业技术学院生物工程系,徐州221006

出　　处：《中国食品添加剂》2015年第3期97-101,共5页China Food Additives

基　　金：徐州生物工程职业技术学院2013年立项科研课题;编号2013B07

摘　　要：利用黑曲霉（Aspergillus niger）XSY0607菌株在小型不锈钢发酵罐中液体发酵生产纤维素酶。分别研究了碳源、氮源、发酵温度、起始pH、发酵时间及接种量等因素对菌株产纤维素酶酶活的影响。确定了黑曲霉XSY0607菌株产纤维素酶的最优条件：以水稻秸秆粉为碳源,以黄豆饼粉为氮源,在温度为30℃,初始p H为5.5,接种量为5%~7%,发酵72h。此条件下黑曲霉XSY0607菌株的CMC酶活为188U/m L,FPA酶活为34U/m L。Aspergillus strain XSY0607 was used in liquid fermentation in a small stainless steel fermentation tank. Study on the carbon source and nitrogen source,fermentation temperature,starting pH value,the amount of fermentation time,inoculation amount and other factors on the effect of cellulase enzyme activity. The optimal conditions for cellulase production Aspergillus XSY0607 strains were ：rice straw powder as carbon source,soybean pie powder as a nitrogen source,temperature 30℃,initial p H 5.5,inoculation amount 5% - 7%,fermentation 72 h.Under the above conditions,CMC activity of strain Aspergillus XSY0607 was 188U/m L,FPA activity was 34U/m L.

关 键 词：黑曲霉 水稻秸秆 纤维素酶 液体发酵 

分 类 号：TS201.3[轻工技术与工程—食品科学]