黑曲霉XSY0607生产柠檬酸培养基优化研究  

Optimization the production of citric acid fermented by Aspergillus niger XSY0607 cellulose

作　　者：孙科[1] 周凤侠 

机构地区：[1]徐州生物工程职业技术学院生物工程系,徐州221006 [2]中央农业广播学校徐州分校,徐州221000

出　　处：《中国食品添加剂》2015年第3期107-112,共6页China Food Additives

基　　金：徐州生物工程职业技术学院2013年立项课题课题编号:2013B07

摘　　要：通过对黑曲霉(Aspergillus niger)XSY0607液体深层发酵柠檬酸的培养基优化试验:通因试验得出黑曲霉XSY0607发酵柠檬酸的最佳碳源为水稻秸秆、最佳有机氮源为蛋白胨、得出如下结论最佳无机氮源NH4NO3、最佳生长因子为牛肉膏。通过Plackett-Burman试验设计得到黑曲霉XSY0607发酵柠檬素的最佳培养基配方为(%):水稻秸秆粉6%、蛋白胨4.5%、NH4NO 30.5%、牛肉膏0.75%、KH2PO4 0.15%、Mg SO44.5×10-3和Fe SO4·7H2O 1.5×10-5。同时通过极差分析还可得出黑曲霉XSY0607发酵培养基中不同成分对柠檬酸发酵的影响顺序为水稻秸秆>蛋白胨>NH4NO3>KH2PO4>Mg SO4>Fe SO4·7H2O>牛肉膏。An optimal process of citric acid produced by submerged fermentation with Aspergillus niger XSY0607 was obtained as follows ：the optimal culture medium was rice straw,peptone,NH4NO3 and beef extract,their functions are carbon source,organic,inorganic nitrogen source and growth factor,respectively. Plackett-Burman design was applied for final culture medium of citric acid submerged fermentation,and the ratio of each material rice straw,peptone,NH4NO3,beef extract,KH2PO4,KH2PO4 and FeSO4· 7H2O is 6%,4.5%,0.5%,0.75%,0.15%,4.5×10^-3 and 1.5×10^-5,respectively. Range analysis showed the influence factors order to the citric acid fermentation from strong to weak was rice straw,peptone,NH4NO3,KH2PO4,MgSO4,Fe SO4· 7H2O and beef extract.

关 键 词：黑曲霉 水稻秸秆 液体深层发酵 

分 类 号：TS202.3[轻工技术与工程—食品科学]