太行菊和野菊不同器官水提液抗氧化活性研究  

Antioxidant Activity of Aqueous Extracts from Different Organs of Opisthopappus Shih

作　　者：魏东伟[1] 徐铭蔓 孙武勇 贾翠英[1] 张晓雯[1] 

机构地区：[1]河南农业大学生命科学学院,郑州450002 [2]河南出入境检验检疫局检验检疫技术中心,郑州450003

出　　处：《中国食品学报》2015年第2期56-63,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：河南省重点科技攻关计划项目(132102110041)

摘　　要：以我国特有植物太行菊和其近缘属的传统药食兼用野菊为试验材料,采用DPPH和ABTS法测定两种菊不同器官水提液的抗氧化活性;分别采用硝酸铝络合分光光度法和Folin-Ciocalteu比色法研究其不同器官的总黄酮与多酚含量。结果表明,太行菊和野菊不同器官水提液均有一定抗氧化活性,其活性与添加量、提取温度与时间有关。太行菊叶和花,野菊花的抗氧化活性较为突出,并且太行菊不同器官水提液的抗氧化活性相当或高于野菊对应器官,在紫外-可见光谱240~400 nm范围出现的黄酮多酚吸收峰值和不同器官的总黄酮和多酚含量一致。两种菊高温短时水提液的抗氧化活性均较高,与紫外-可见光谱特性一致。菊花除头状花序外,叶与茎也是可利用的部位。太行菊比近缘属的传统药用野菊具有更好的保健和药用功效,其整个植株的开发利用潜力显著。Antioxidant activity evaluation by ABTS and DPPH scavenging assays and the corresponding UV-vis spectra of aqueous extracts from Opisthopappus taihangensis（Ling） Shih（Opisthopappus）, endemic to China, and its Relatives genera traditional medicine Dendranthema indicum（C. indicum） were measured. Moreover, total flavonoid and phenolic contents were determined by aluminum nitrate colorimetric method and Folin-Ciocalteu method respectively. It was found that aqueous extracts from different organs of Opisthopappus and C. indicum possessed some antioxidant activities,related with the extraction concentrations, extraction temperatures and times. Antioxidant activities of leaves and flowers of Opisthopappus, and the flower of C. indicum was prominent, and the activities of aqueous extracts from different organs of Opisthopappus also higher than that of corresponding organs of C. indicum, consistent with their corresponding UV-vis absorption peaks of flavonoids polyphenols within 250 ~350 nm and the result of total flavonoids and polyphenols content analysis. In addition, the antioxidant activity of aqueous extracts at 90 ℃ 20 min was distinguished, compatible with their corresponding UV-vis absorption peak, it was demonstrated that the tradition of drinking chrysanthemum tea brewed with boiling water in China has some basis. Therefore, the leaves and stems of Chrysanthemum besides its flowers are available parts, Opisthopappus may be much better for health-promoting and medicinal properties than Relatives genera C.indicum. Huge utilization potential is expected from the whole plant of Opisthopappus.

关 键 词：太行菊 野菊 水提液 抗氧化活性 多酚 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]