气相色谱-燃烧-同位素比率质谱法测定葡萄酒中5种挥发性组分的碳同位素比值及其在产地溯源中的应用  

Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry for Determination of Five Volatile Components in Wine and Its Application in Geographical Origin Traceability

作　　者：吴浩[1,2,3] 谢丽琪[1,2] 靳保辉[1,2] 颜治[1,2] 陈波[1,2] 华红慧[1,2] 林光辉[3,4] 

机构地区：[1]深圳出入境检验检疫局食品检验检疫技术中心,深圳518016 [2]深圳市检验检疫科学研究院食品安全检测技术研发重点实验室,深圳518016 [3]清华大学深圳研究生院海洋科学与技术学部,深圳518055 [4]清华大学地球系统科学研究中心,北京100084

出　　处：《分析化学》2015年第3期344-349,共6页Chinese Journal of Analytical Chemistry

基　　金：深圳市技术开发项目(No.CXZZ20120831160213590);国家质检总局科技计划项目(Nos.2012IK189;2013IK180);深圳出入境检验检疫局科技创新项目(No.SZ2013101)资助

摘　　要：建立了一种利用气相色谱-燃烧-同位素质谱（Gas chromatography-combustion-Isotope ratio mass spectrometers,GC-C-IRMS）测定葡萄酒中5种挥发性组分（即乙醇、丙三醇、乙酸、乳酸乙酯、2-甲基-丁醇）碳稳定同位素比值新方法。优化了GC-C-IRMS测定条件,进样量小于0.5μL,样品分析时间小于14 min。对以上5种挥发性成分标准品的测定精密度为0.08‰-0.25‰,葡萄酒样品的测定精密度为0.09‰-0.36‰,与元素分析-同位素比率质谱仪（Element analyzer-isotope ratio mass spectrometers,EA-IRMS）比较,其测定偏差低于0.5‰。利用该技术分析了产自法国、澳大利亚、美国和中国共54支葡萄酒中5种挥发性组分的碳稳定同位素值并进行产地溯源分析,判别分析（Discriminant analysis,DA）结果表明,仅利用以上5种挥发性组分的碳同位素比值就能有效区分以上4个产地的葡萄酒,说明葡萄酒挥发性成分稳定碳同位素可应用于葡萄酒的产地溯源。A method was developed for analyzing the stable carbon isotope ratio of five volatile components（ Ethanol,Glycerol,Acetic acid,Ethyl lactate,2-methyl-butanol） in wine using gas chromatographycombustion-isotope ratio mass spectrometer（ GC-C-IRMS）. The sample injection volume was less than0. 5 μL,and the analytical time of each run was less than 14 min. The precision of this method was 0. 08‰-0. 25‰ for analyzing standards,while 0. 09‰- 0. 36‰ for wine samples. Compared to element analysisisotope ratio mass spectrometry（ EA-IRMS） results,the deviations were lower than 0. 5‰. Fifty-four wine samples from France,Australia,America and China were considered. The δ13C of five volatile components were measured using GC-C-IRMS. Discriminant analysis（ DA） was employed for analyzing the geographical origin traceability of selected wine. The result indicated that δ13C of volatile components could be used to distinguish the origin of wines. The method was shown to be effective in improving detection of the origin traceability of wine.

关 键 词：产地溯源 同位素 香气成分 葡萄酒 气相色谱-燃烧-同位素比率质谱仪 

分 类 号：TS262.6[轻工技术与工程—发酵工程] O657.63[轻工技术与工程—食品科学与工程]