固定化金属亲和层析富集麦胚蛋白金属螯合肽的研究  

Enrichment of Metal Chelating Peptides from Wheat Germ Protein by Immobilized- Metal Ion Affinity Chromatography

作　　者：王晓萍[1] 郭晓娜[1] 朱科学[1] 彭伟[1] 周惠明[1] 

机构地区：[1]江南大学食品学院,无锡214122

出　　处：《中国粮油学报》2015年第3期101-105,共5页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金(31101384);江苏省自然科学基金(BK2011041)

摘　　要：利用3种蛋白酶（碱性蛋白酶Alcalase 2.4L、风味蛋白酶Flavourzyme 500MG和木瓜蛋白酶Papain）分别对麦胚蛋白进行酶解,筛选金属螯合率最高的酶解产物,再利用固定化金属亲和层析富集金属螯合肽,并分析金属螯合肽的相对分子质量分布和氨基酸组成。结果表明：在Alcalase 2.4L的酶解作用下,酶解时间200 min时,酶解产物的金属螯合率达到最大值（69.62±0.96）%。以此麦胚蛋白酶解物通过固定化金属离子亲和层析富集得到的组分,相对分子质量集中在180-2 000,谷氨酸（Glu）和天冬氨酸（Asp）的含量分别高达（22.64±1.50）%和（14.93±0.24）%。Wheat germ protein has been hydrolyzed by three proteases（ Alcalase 2. 4L,Flavourzyme 500 MG and Papain） respectively. The hydrolysates with highest metal chelating ability have been injected into immobilized-metal ion affinity chromatography to enrich metal chelating peptides. The relative molecular mass distribution and amino acid composition of the metal chelating peptides were analyzed. The results showed that the hydrolysates could be obtained on condition as Alcalase 2. 4L and 200 min,and the highest metal chelating ability reached（ 69. 62 ±0. 96） %. The relative molecular mass of metal chelating peptides separated from wheat germ protein hydrolysates was concentrated in the range of 180 - 2 000. Besides,the metal chelating peptides had high content of Glu and Asp with the level of（ 22. 64 ± 1. 50） % and（ 14. 93 ± 0. 24） % respectively.

关 键 词：麦胚蛋白 金属螯合肽 固定化金属亲和层析 相对分子质量 氨基酸组成 

分 类 号：TS209[轻工技术与工程—食品科学]