龙眼壳黄酮的微波提取及体外抗氧化活性研究  

Microwave-assisted Extraction of Flavonoids from Longan Pericarp and Its Antioxidant Activity Study

作　　者：刘焕云[1] 王海燕[1] 梁燕[1] 

机构地区：[1]河北经贸大学生物科学与工程学院,石家庄050061

出　　处：《天然产物研究与开发》2015年第3期438-441,458,共5页Natural Product Research and Development

基　　金：河北省教育厅科学研究计划(Z2009422)

摘　　要：研究了微波辅助提取龙眼壳黄酮的工艺条件,并对其进行了抗氧化性能的研究。正交试验结果表明较佳提取工艺为:V(70%乙醇)∶m(龙眼壳粉)=30 m L∶1 g,微波功率480 W处理20 s后,于水浴30℃浸提30min,龙眼壳黄酮得率为3.465%。抗氧化试验表明龙眼壳黄酮对亚硝酸盐具有良好的清除能力,其效果优于Vc,对卵黄脂蛋白脂质过氧化及羟基自由基具有明显的抑制作用,其效果分别弱于对照物2,6-二叔丁基-4-甲基苯酚(BHT)和Vc。Flavonoids was extracted from Longan Pericarp by microwave-assisted method and its antioxidant activities were measured. The conditions of extraction were optimized by orthogonal test design L9（ 34）. The results showed the optimum conditions as follows： Longan Pericarp（ 1 g） was soaked in 30 m L of 70% ethanol at 30 ℃ water bath for 30 min after being dealt with 480 W microwave for 20 s. The flavonoids yield was 3. 465%. Then its antioxidant activity was evaluated by three different assays in vitro. Comparing with the scavenging ability of Vc,it had better function to nitrite system. However the inhibition of the extract to lipid peroxidation of yolk lipoprotein and hydroxyl radical was lower than that of butylated hydroxytoluene（ BHT） and Vc,respectively.

关 键 词：龙眼壳 微波辅助提取 黄酮 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]