石榴花多糖醇提工艺的响应曲面法优化及其抗氧化活性研究  

Study on optimization of extraction conditions for polysaccharide from pomegranate flowers and its antioxidant activities

作　　者：柯春林[1] 李作美[1] 路滨宇 钱时权[1] 张斌[1] 袁静[1] 

机构地区：[1]蚌埠学院生物与食品工程系,安徽蚌埠233030 [2]复旦大学药学院,上海201203

出　　处：《食品工业科技》2015年第8期286-289,304,共5页Science and Technology of Food Industry

基　　金：安徽省自然科学基金项目(1408085MH209);安徽省高等学校省级自然科学基金项目(KJ2012B094);安徽省大学创业训练计划项目(AH20141305065)

摘　　要：以石榴花为原料,用热水浸提法提取其多糖。在单因素实验的基础上,运用Box-Behnken实验设计,采用响应曲面法对石榴花多糖的醇提条件进行优化。结果表明最佳工艺为:提取时间4h,温度76℃,料液比1∶52,在此基础上进行验证实验,实测多糖得率为6.35%。同时,不同的体外抗氧化实验结果表明石榴花多糖具有适度的还原力,较强的清除亚硝酸盐(NO2-)和超氧自由基(O2-)的清除效果,较好的螯合金属离子能力,而且石榴花多糖的抗氧化活性随着多糖浓度的提高而增强。Pomegranate flowers as row material were used for extraction polysaccharide with hot water. On the basis of single factor experiments,the optimized extraction conditions for polysaccharides were determined by using response surface methodology through the Box-Behnken design. The optional conditions were as follows：extraction time 4 hours,temperature 76℃,ratio of raw material to solvent 1∶52. Under the conditions,the yield of6.35% was obtained by the verification experiment. And the polysaccharides from pomegranate flowers showed moderate reducing power,strong clearance rate of nitrite（NO2-） and super oxygen free radical（O2-）and the ability to chelate metal ions,and the antioxidant activity of polysaccharides increased with the improvement of the polysaccharide concentration.

关 键 词：石榴花 多糖 响应曲面 提取优化 抗氧化活性 

分 类 号：TS281.1[轻工技术与工程—食品科学与工程]