鸡贫血病毒广州株VP3和VP1基因的克隆与序列分析  

Cloning and Nucleotide Sequence Analysis of VP3 and VP1 Gene Sequences of Three CAV Isolates from Guangzhou

作　　者：沈海燕[1] 张建峰[1] 刘志成[1] 周恒[1,2] 郭翠丽[1,2] 郭鹏举[1] 张春红[1] 

机构地区：[1]广东省农业科学院动物卫生研究所广东省兽医公共卫生公共实验室广东省畜禽疫病防治研究重点实验室,广东广州510640 [2]华南农业大学兽医学院,广东广州510642

出　　处：《广东畜牧兽医科技》2015年第2期25-28,共4页Guangdong Journal of Animal and Veterinary Science

基　　金：国家科技计划项目(2014DFA31730);广东省科技计划项目(2011B050700007;2012B050500013);广东省农业科学院院长基金(201114)

摘　　要：采用PCR方法成功克隆了3株鸡贫血病病毒（CAr）广州株VP3和VPl的部分基因，并进行了核苷酸序列测定。序列分析表明，3株CAV广州株与国内外其他21株CAV毒株的核苷酸序列相似性在89．5％-100．0％之间；推导氨基酸序列的相似性在84．8％-98．2％之间。系统发育进化树分析显示，3株CAV广州株和中国TJBD40、SD22、SD24株都同处于一个分支上，而与马来西亚的SMSC-1株、日本的G6株以及澳大利亚的CAU269／7株的亲缘关系较远。The partial VP3 and VP1 genes of three CAV isolates from Guangzhou were cloned through PCR and sequenced. Sequence comparison revealed 89.5% to 100.0% similarities at nucleotides level and 84.8% to 98.2% similarities at amino acids level among the three CAV isolates from Guangzhou and other twenty-one CAV genomic sequences from GenBank. Phylogenetic tree analysis showed that the three CAV isolates from Guangzhou were genetically close to TJBD40, SD22, and SD24 isolated from China, but they were far away from Malaysia SMSC-1 isolate, Japan G6 isolate andAustralian CAU269/7 isolate.

关 键 词：鸡贫血病病毒 核苷酸序列 分子进化树 

分 类 号：S854.43[农业科学—临床兽医学]