贵州省3个少数民族HLA-G基因14bp插入/缺失的多态性研究  

Analysis of the 14 bp Insertion/Deletion Polymorphism in Human Leukocyte Antigen-G Gene in Chinese Buyi,Shui,and Miao Ethnic Groups in Guizhou Province

作　　者：何燕[1] 赵孝梅[1] 左娅[1] 张婷[1] 邓婕[1] 吴昌学[1] 禹文峰[1] 官志忠[1] 

机构地区：[1]贵阳医学院分子生物学重点实验室,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第3期217-221,共5页Journal of Guiyang Medical College

基　　金：国家"十二.五"科技支撑计划项目(No:2013BAI05B03);贵州省科技厅社会发展攻关项目[No:SY(2012)3135]

摘　　要：目的:了解贵州省3个少数民族(布依族、水族、苗族)群体人类白细胞抗原-G(HLA-G)基因14 bp插入/缺失多态性的分布规律及其与遗传背景之间的关系。方法:采用PCR扩增、电泳及测序的方法对来自贵州地区的布依族、水族、苗族共344例健康个体DNA进行HLA-G基因14 bp插入/缺失多态性检测,并与文献报道的仡佬族、壮族等13个民族群体的HLA-G基因14 bp插入/缺失多态性分布数据进行对比。结果:布依族、水族和苗族人群的+14 bp等位基因频率分别为23.7%、26.0%和38.1%,+14 bp/+14 bp基因型频率分别为9.40%、10.4%和15.9%,14 bp插入频率比较示布依族和苗族差异有统计学意义(χ2=11.345,P=0.001),苗族与水族差异有统计学意义(χ2=6.125,P=0.009)而布依族和水族间比较差异无统计学意义(P>0.017);与其他人群数据比较,苗族群体的14 bp插入/缺失分布与其他人群相似,而作为壮侗语族的布依族、水族则有自己独特的14 bp插入/缺失分布规律。结论:布依族、水族人群的14 bp插入/缺失分布相似,但是与苗族人群的分布存在一定的差异。Objective： To investigate the distribution of HLA-G gene 14 bp insertion /deletion polymorphism in Buyi, Shui and Gelao populations and their relationship with genetic background.Methods： PCR amplification,agarose gel electrophoresis and sequencing were used to explore the distribution of 14 bp insertion/deletion in HLA-G gene in 344 healthy people of these three ethnic groups,and the data were compared with that of 13 ethnic groups such as Gelao,Zuang reported in the literature. Results： The frequencies of ＋ 14 bp in Buyi,Shui and Miao were 23. 7%,26. 0% and 38. 1%respectively. The genotype frequencies of ＋ 14 bp/＋ 14 bp in Buyi,Shui and Miao were 9. 40%,10. 4% and 15. 9% respectively. The differences of 14 bp insertion frequencies between Buyi and Miao,between Miao and Shui were statistically significant（ P 0.〈05）,while no significant difference was found between Buyi and Shui（ P〉0. 05）. When compared with other groups,the distribution characteristic of ＋ 14 bp insertion/deletion in Miao was similar to them,but Buyi and Shui showed significance difference from others. Conclusion： The distribution characteristics of ＋ 14 bp insertion/deletion in Buyi is similar to Shui,but they are different from that of Miao.

关 键 词：基因 HLA-G 14 bp插入/缺失多态 布依族 水族 苗族 贵州 

分 类 号：R394[医药卫生—医学遗传学] R34-33[医药卫生—基础医学]