营养肉汤中不同因素影响下金黄色葡萄球菌生长模型的构建  

Modeling Staphylococcus aureus Growth as a Function of Different Conditions in Nutrient Broth

作　　者：李金春[1] 周彤[1] 李家鹏[1] 陈曦[1] 杨君娜[1] 许随根 陈文华[1] 

机构地区：[1]中国肉类食品综合研究中心,肉类加工技术北京市重点实验室,北京100068

出　　处：《肉类研究》2015年第3期5-10,共6页Meat Research

基　　金：国家高技术研究发展计划(863计划)项目(2011AA100805)

摘　　要：采用营养肉汤培养基,研究不同温度、pH值和水分活度对金黄色葡萄球菌生长规律的影响,并构建金黄色葡萄球菌菌落数的一级生长模型和最大比生长速率、延滞期的二级生长模型,其中一级模型通过Baranyi模型来拟合;二级模型包括单因素二级模型和多因素二级模型,分别通过Belehradek平方根模型和二次多项式回归模型来拟合。结果表明:金黄色葡萄球菌生长速率随着温度、PH值和水分活度的增加而增加,而延滞期随着温度、PH值和水分活度的增加而降低,并且温度与PH值,温度与水分活度以及PH值与水分活度之间存在明显的交互作用。一级Baranyi模型、二级平方根模型以及多项式回归模型拟合性较好(R^2>0.85)。通过数学检验参数精确因子(A_f)、偏差因子(B_f)、均方根误差(RMSE)对模型进行数学检验,表明构建的一级、二级模型适用性、可靠性和拟合性均在可接受范围内,在建模设定的范围内能预测金黄色葡萄球菌建模范围内的延滞期和最大比生长速率。Effects of temperature, pH, and water activity （aw） on the growth of Staphylococcus aureus in nutrient broth medium were studied, respectively, and the first-order kinetics models of bacterial count （CFU） and the second-order kinetics models of maximum specific growth rate （μmax） and lag time were built subsequently. Results showed that increasing temperature, pH and aw could increase the μmax but decrease the value of lag time. Colony-forming units （CFU） of S. aureus were modeled as a function of T, pH and aw, which revealed a good relationship as evaluated by the Baranyi model. The single-variable second-order models were evaluated by Belehradek square-root-type model with correlation coefficients more than 0.85. The multi-variable second-order models, namely polynomial logistic regression equations, were fitted using a stepwise method with correlation coefficient more than 0.85. Then, the goodness of fit of the established models was evaluated by accuracy factor （Af）, bias factor （Bf） and root mean square error （RMSE）. It was found that their applicability, reliability and goodness of fit were within acceptable ranges. The first-order and second-order models provide a useful and accurate approach to predicting the growth of S. aureus within certain limits.

关 键 词：金黄色葡萄球菌 营养肉汤 预测微生物学 生长模型 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]