野生巨大口蘑1株新菌株ITS鉴定及菌丝培养基优化  

Identification of a new strain of Tricholoma giganteum by ITS and optimization of culture medium for mycelium

作　　者：马紫英[1] 倪焱[1] 魏要武[1] 聂健[1] 杨水莲[1] 昌毓嵩 莫美华[1] 

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《华南农业大学学报》2015年第3期98-103,共6页Journal of South China Agricultural University

基　　金：国家自然科学基金(31272218);国家星火计划项目(2012GA780042;2013GA780040);广东省省部产学研项目(2012B091100302);广东省农业科技推广专项(201201138);广州市花都区科技计划项目(HD13CXY-010)

摘　　要：【目的】鉴定1株巨大口蘑Tricholoma giganteum新的野生菌株，并优化其菌丝的最适培养基．【方法】以1株野生巨大口蘑为研究对象，采用组织分离法，从野生巨大口蘑子实体中分离纯化得到纯菌丝，利用分子生物学方法进行鉴定，通过测定菌丝生长速度、菌丝长势等指标对其进行培养条件研究，并运用均匀设计法对培养基进行优化，采用二次多项式逐步回归方法对试验结果进行分析．【结果和结论】该野生菌株经鉴定为巨大口蘑，GeneBank编号为JXl93694，命名为SCAU3，其菌丝生长的最适温度为30～32℃，最适pH为7～8，最佳碳源为麦芽糖，最佳氮源为酵母膏．经均匀设计法优化得到最适培养基组合：麦芽糖26g·L^-1，酵母膏2g·L^-1，MgSO42．7g·L^-1，KH，P041．8g·L^-1，维生素Bl 16mg．L^-1，pH7．7．【Objective】A new wild strain of Tricholoma giganteum was identified and the culture medium of mycelium was optimized. 【Method】The pure mycelium of T. giganteum was obtained from the wild fruitbody by the tissue isolation and identified by molecular biological techniques. The culture condition of the mycelium was researched by measuring mycelial growth rate and growth vigor. The optimum growth medium of mycelium was determined by the uniform-design-method. The method of quadratic polynomial step regression analysis was employed for analyzing the experiment results. 【Result and conclusion】The wild strain was identified as T. giganteum,named as SCAU3. The accession number of ITS sequence was JX193694 in Gene Bank. The results show that the optimum carbon source and nitrogen source for mycelium growth are maltose and yeast extract respectively. The optimum temperature is 30- 32 ℃,and the optimum p H in medium was 7- 8. The optimum medium for mycelial growth is maltose 26 g·L- 1,yeast extract 2 g·L- 1,Mg SO42. 7 g·L- 1,KH2PO41. 8 g·L- 1,vitamin B1 16 mg·L- 1,p H7. 7.

关 键 词：巨大口蘑 ITS鉴定 培养基优化 均匀设计 温度 pH 

分 类 号：S511[农业科学—作物学] S502