东方伊萨酵母生物转化法制备胞二磷胆碱  

Biological Preparation of CDP-C by Issatchenkiaorientalis CCTCCNO:M2011272

作　　者：宋丽芳[1,2] 尤翠萍[1,2] 陶冠军[3] 张梁[1,2] 石贵阳[1,2] 

机构地区：[1]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [3]食品科学与技术国家重点实验室,江南大学,江苏无锡214122

出　　处：《食品与生物技术学报》2015年第2期201-208,共8页Journal of Food Science and Biotechnology

基　　金：教育部“新世纪优秀人才支持计划”项目(NCET-11-0665)

摘　　要：以5'-胞苷酸和磷酸胆碱钙为底物,利用东方伊萨酵母生物转化生成胞二磷胆碱,并对转化条件进行优化,得到最优的转化条件(胞苷酸17.5g/L,磷酸胆碱钙12.5g/L,磷酸氢二钾12.5g/L,硫酸镁3.0g/L,硫酸锰0.6g/L,葡萄糖50g/L,甲苯30mL/L,酵母泥375g/L,转化液pH8.0),胞二磷胆碱产量可达10g/L以上,最高可达11.592g/L。对转化液去细胞、去蛋白质,717离子交换树脂吸附胞二磷胆碱,以0.05mol/LNaCl洗脱,可以得到纯度在98%以上的胞二磷胆碱。5＇-cytidine acid and choline calcium phosphate as the substrates were converted into CDP-C by Issatchenkiaorientalis.The optimal conversion condition was as follows：CMP17.5g/L,choline calcium phosphate 12.5g/L,potassium hydrogen phosphate 12.5g/L,seven hydrated magnesium sulfate 3.0g/L,manganese sulfate 0.6g/L,glucose 50g/L,toluene 30 m L/L,Yeast mud375g/L,and the pH of convert liquid 8,the output of CDP-C can reach more than 10g/L,up to11.592g/L.The conversion solution was decellularized,deproteinized and the CDP-C was obtained by eluting 717 ion exchange resin with 0.05 M Na Cl,whose purity was more than 98%.

关 键 词：东方伊萨酵母 转化条件 离子交换树脂 胞二磷胆碱 

分 类 号：TQ929[轻工技术与工程—发酵工程]