黑曲霉木聚糖酶曲盘发酵及其粗酶性质研究  

Solid State Fermentation of Xylanase from Aspergillus niger and Property of Crude Enzyme

作　　者：周晨妍[1] 杜晓娜[2] 王丹丹[2] 刘振华[1] 王燕[1] 

机构地区：[1]新乡医学院生命科学技术学院,河南新乡453003 [2]新乡医学院三全学院,河南新乡453003

出　　处：《贵州农业科学》2015年第2期90-94,共5页Guizhou Agricultural Sciences

基　　金：河南省教育厅科学技术研究重点项目"黑曲霉XZ-3S木聚糖酶基因克隆及工程菌的选育"(13A180861);河南省高等学校青年骨干教师资助计划项目"黑曲霉XZ-3S所产木聚糖酶基因工程菌的选育及其发酵工艺研究"(2011GGJS-125);新乡医学院科研项目培育基金项目"提高黑曲霉XZ-3S木聚糖酶热稳定性的定向分子改造研究"(2013ZD113)

摘　　要：为研究黑曲霉菌株（Aspergillus niger）XZ-3S曲盘发酵木聚糖酶条件及所产粗酶的性质,采用曲盘（大小φ20cm×4cm）进行固态发酵条件研究,并对所产粗酶进行酶学性质测定。结果表明：在培养基干料100g、初始料水比1∶1.7、初始pH自然状态、相对干物料接种量10%、中间定时翻曲补水和28℃连续培养60h条件下,酶活力最高可达24 380IU/g干曲;粗酶最适反应pH5.0,最适温度50℃;温度低于50℃、pH3.0~8.0的条件下酶较稳定;EDTA（活性物质）、Cu2＋、Co2＋对酶有明显的激活作用,Pb2＋、Mg2＋、Fe2＋、Mn2＋、Ca2＋、Fe3＋、Al 3＋对酶有不同程度的抑制作用。The solid state fermentation condition of xylanase from A.niger XZ-3S strain and property of the produced crude enzyme were studied and determined by tray culture and enzyme activity method. The results showed that the xylanase activity can reach 24 380 IU/g under the fermentation conditions of 100g dry substance,1 ∶ 1.7 of dry substance ：water,natural initial pH,10% inoculation size of dry substance,timing stirring and watering and 28℃ for 60h,and the optimal pH and temperature for the produced crude enzyme is 5.0 and 50℃ respectively.The property of the crude enzyme is stable under the conditions of below 50℃ and pH3.0~8.0.EDTA,Cu2＋ and Co2＋ have the obvious activation effect on activity of xylanase but Pb2＋ ,Mg2＋ ,Fe2＋ ,Mn2＋ ,Ca2＋ ,Fe3＋ and Al3＋ have different inhibition effect on activity of xylanase to varying degrees respectively.

关 键 词：木聚糖酶 黑曲霉 固态发酵 酶学性质 

分 类 号：S182[农业科学—农业基础科学]