大曲中产香兼性厌氧细菌的筛选及挥发性成分分析  

Screening of facultative anaerobic bacteria from Luzhou-flavor Daqu and determination of its volatile constituents

作　　者：明红梅[1] 余欢[2] 周健[1] 郭志[1] 许德富 姚霞[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]重庆邮电大学移通学院,重庆合川401520 [3]泸州老窖股份公司,四川泸州646000

出　　处：《食品与机械》2015年第2期7-10,共4页Food and Machinery

基　　金：四川省高等教育质量工程项目(编号:2011-659);泸州市科技局应用基础项目(编号:2011-S-28);酿酒生物技术及应用四川省重点实验室项目(编号:NJ2011-13);泸州老窖科研奖学金项目(编号:13ljzk04)

摘　　要：从浓香型大曲中分离筛选兼性厌氧细菌,研究该类菌在制曲工艺环境中种群分布的可行性,并进一步探索该类群微生物的产香特性及其挥发性组分,为提高大曲质量奠定基础。采用二氧化碳培养法对大曲样品进行菌株的筛选及分离纯化,最终获得9株兼性厌氧细菌。其中编号为X3菌株产香性能突出,经初步鉴定属于棒状杆菌属。进一步通过顶空固相微萃取—气相色谱—质谱联用分析X3菌株固态发酵产物的挥发性物质,结果表明,其挥发性物质中的吡嗪类物质、苯乙醇、愈创木酚、苯甲醛等,与浓香型大曲产香物质相符。The facultative anaerobic bacteria in Luzhou-flavor Daqu were isolated and screened to study the species distribution of this kind of bacteria in the fermentation environment of Daqu.Then,the screened bacteria were further researched for the formation ability of the flavor components and their volatile constituents in order to improve the quality of Daqu.Through separation and purification of microorganism in the samples of Daqu by Candle jar method,9 facultative anaerobic bacteria were obtained.Among them,the one which was coded as X3 provided with the prominent formation ability of the flavor components and was assigned as Corynebacterium with preliminary identification.Through analyzing the volatile components in the products by solid-state fermentation with HS—SPME—GC—MS,it showed that some volatile substances such as pyrazines,phenethyl alcohol,ethyl phenylacetate and guaiacol were be accord with Luzhou-flavor Daqu.

关 键 词：浓香型大曲 兼性厌氧细菌 筛选 挥发性成分 

分 类 号：TS261.1[轻工技术与工程—发酵工程]