方格星虫蛋白酶解工艺优化  

Study on hydrolysis conditions of Sipunculus nudus by response surface methodology

作　　者：孙雪萍[1] 杨家林[1] 徐艳[1] 刘海娟[1] 任义[2] 

机构地区：[1]广西海洋研究所,广西北海536000 [2]北海艺术设计职业学院环境艺术设计系,广西北海536000

出　　处：《食品与机械》2015年第2期238-242,共5页Food and Machinery

基　　金：广西科学院基本科研业务费资助项目(编号:13YJ22HYS15);广西海洋生物技术重点实验室开放基金资助课题(编号:GLMBT-201407)

摘　　要：以水解度为指标,比较7种蛋白酶对方格星虫的水解效果。在单因素试验考察的基础上,采用Box-Behnken模型响应面设计,建立方格星虫蛋白酶解的二次回归模型,确定最佳的水解条件。结果表明,复合蛋白酶对方格星虫蛋白的水解效果最佳,最佳水解条件为:酶与底物比为7.8%,酶解时间为4 h,酶解温度为50℃。该条件下,方格星虫蛋白的水解度达到24.81%。Using DH（degree of hydrolysis） as the main index,hydrolysis effects on Sipunculus nudus protein of seven proteases were compared.On the basis of single factor experiment,response surface methodology and Box-Behnken model were employed to establish the quadratic model for optimization the hydrolysis conditions.Results showed that the DH of Protamex was higher than those of others.The optimum hydrolysis conditions were as follows： the ratio of enzyme to substrate of 7.8%,hydrolysis time of 4 h and the hydrolysis temperature at 50 ℃.Under this condition,DH reached 24.81%.This study was aimed to provide theoretical basis for the development and utilization of S.nudus.

关 键 词：方格星虫 蛋白质 酶解 水解度 

分 类 号：TS201.21[轻工技术与工程—食品科学]