银杏愈伤组织黄酮提取条件的正交实验优化  

Optimization of extracting conditions of flavonoids from Gingko Callus by orthogonal experiment

作　　者：梁文惠[1] 黄翔[1] 林丽春[1] 夏年益 张珣[1] 彭春林[1] 丘添 施晓宇[1] 邓祥宜[1] 

机构地区：[1]华中农业大学楚天学院食品与生物科技学院,湖北武汉430205

出　　处：《食品工业科技》2015年第9期209-213,共5页Science and Technology of Food Industry

基　　金：国家级大学生科技创新项目(201314035001);校级大学生科技创新项目(201201)

摘　　要：以银杏愈伤组织为材料,对银杏黄酮的提取条件进行优化。通过单因素实验研究乙醇浓度、液料比、微波功率、微波时间、水浴温度、水浴时间等对黄酮得率的影响,并进行了正交实验优化。正交实验的结果表明:以40%乙醇为提取溶剂,液料比25∶1,在中火档(528W)微波60s,不进行水浴处理,黄酮得率达到20.92mg/g。Using Ginkgo callus as material,the optimization of the extraction conditions of Ginkgo flavonoids was done.Through single- factor tests,the influences of several factors on extraction rate of Ginkgo flavonoids were investigated,such as ethanol concentration,liquid- powder ratio,microwave power,microwave processing time,water bath temperature,water bath processing time,etc. Then,orthogonal test was carried out. The results of orthogonal experiment indicated that： using 40% ethanol as the extraction solvent,liquid- powder ratio 25 ∶ 1,microwave processing 60 s under middle power level（ 528W）,no water bath treatment,the extraction rate of flavonoids reached 20.92 mg / g.

关 键 词：银杏愈伤组织 黄酮 提取条件优化 正交实验 微波处理 

分 类 号：TS205[轻工技术与工程—食品科学]