β-环糊精辅助提取茶末总黄酮的工艺优化  

Optimizing β-CD Assisted Extraction of Total Flavonoids from Tea Dust

作　　者：冯欢欢[1] 陈识文 高梦祥[1] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025

出　　处：《湖北农业科学》2015年第4期935-938,943,共5页Hubei Agricultural Sciences

基　　金：湖北省自然科学基金项目(2013CFB392)

摘　　要：以制茶副产物茶末为原料,采用β-环糊精辅助提取茶末总黄酮,在单因素试验基础上,采用BoxBehnken试验设计和响应面分析法研究了料液比(g∶m L)、β-环糊精含量和提取温度对茶末总黄酮提取率的影响,并建立该工艺的二次多项式模型。结果表明,回归模型具有极显著性,可以对茶末总黄酮提取率进行很好地分析和预测;确定了茶末总黄酮提取工艺的最佳条件为料液比1∶61.83(g∶m L)、β-环糊精含量8.27%、温度53℃,且在此条件下验证茶末总黄酮提取率为7.843%,接近预测值7.835%。与传统的水回流法和超微粉碎法相比,β-环糊精辅助提取法高效环保,且提取率更高。The β-CD assisted extraction was used to extract total flavonoids from by-products of tea dust-tea. Based on the single factor experiment, the effects of material-to-solvent ratio,β-CD content and extraction temperature on the extraction efficiency of total flavonoids were investigated with the Box-Behnken experiment and the response surface analysis. A quadrat- ic polynomial model was established and verified. The optimal conditions of extracting total flavonoids were solid-to-solvent ratio of 1：61.83 （g： mL）, β-CD content of 8.27%, extraction temperature of 53℃. Under these conditions, the extraction efficiency of total flavonoids was 7.843%, close to the predicted value of 7.835%. Compared with the traditional boiling reflux method and ultrafine grinding extraction technology, the β-CD assisted extraction was efficient, environment friendly with high extraction rate.

关 键 词：茶末 总黄酮 Β-环糊精 提取 响应面法 

分 类 号：S571.1[农业科学—茶叶生产加工] TS272[农业科学—作物学]