地衣芽孢杆菌发酵柞蚕丝胶蛋白生产丝胶肽工艺优化  

Process Optimization on Sericin of Tussore to Produce Silk Sericin Peptide by Bacillus licheniformis Fermented

作　　者：孟详迪 张萱[2] 马万红[1] 郭志富[2] 张良[2] 

机构地区：[1]东北育才学校高中部,辽宁沈阳110179 [2]沈阳农业大学生物科学技术学院,辽宁沈阳110161

出　　处：《园艺与种苗》2015年第3期45-48,共4页Horticulture & Seed

摘　　要：[目的]柞蚕结茧产生的茧壳中的丝胶利用率不高,利用地衣芽孢杆菌降解丝胶蛋白,为解决丝胶废弃物再利用问题提供有效方法。[方法]以柞蚕茧壳为研究材料,利用地衣芽孢杆菌进行发酵,以辐射梯度、发酵天数、温度、转速等为因素,在单因素试验的基础上,以水解度为指标,通过组合试验设计对发酵条件进行优化,获得最佳发酵条件。[结果]最佳发酵条件为:辐射梯度15 k·gy、发酵天数5 d、摇床转速160 r/min。在该条件下,得到最大水解度为72.284%。[结论]该研究为柞蚕茧壳丝胶再利用提供了有效方法,为柞蚕茧壳生产丝胶肽提供了理论基础。Objective] Utilization of silk sericin in tussore cocoon was not high. Bacillus licheniformis could degrade silk sericin, which was the effective way to solve silk sericin reusing. [Method] To gain best fermentation condition, Bacillus licheniformis were used to ferment tussore cocoon and optimize irradiation grads, fermentation days, temperature of fermentation, rotate speed etc. According to combination experimental design and single factor test, the fermentation was optimized. [Result] Best fermentation condition：irradiation grads was 15 k·gy, fermentation days was 5 days and rotate speed was160 r/min. The best degree of hydrolysis was 72.284%. [Conclusion] This study provided the effective way and theoretical basis for production of silk sericin peptide with using tussore cocoon.

关 键 词：柞蚕 茧壳 发酵 丝胶肽 

分 类 号：S886.9[农业科学—特种经济动物饲养]