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作 者:王勇[1,2] 张锦玲 张立明[1,2] 郭鹏[1,2] 袁丽佳[1] 刘大程[1,2]
机构地区:[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]农业部动物临床诊疗技术重点实验室,内蒙古呼和浩特010018
出 处:《中国酿造》2015年第4期66-71,共6页China Brewing
基 金:国家自然科学基金项目(31260560);内蒙古科技厅专利转化项目(20140185);内蒙古农业大学科技成果转化项目(CGZH2013007)
摘 要:对复合酵母菌固态发酵动力学模型进行研究,并通过干重法确定发酵过程菌体生物量。采用Logistic方程,Luedeking—Piret方程和Luedeking-Piret/like方程进行最优参数估计和非线性拟合,得到了描述发酵过程中菌体生长、β-葡聚糖和甘露聚糖合成、底物总糖消耗的动力学模型,通过对Luedeking-Piret模型的计算,可知β-葡聚糖和甘露聚糖合成与菌体合成是偶联的。对试验数据与模型预测值进行比较,发现平均相对误差都小于10%,说明模型反映了复合酵母菌发酵过程的动力学特征,表明该动力学模型对指导生产具有实际意义。The kinetics model of solid state fermentation by compound yeast was studied and the biomass was determined based on dry weight variation. The optimal parameters were estimated and nonlinear fitting was performed by logistic model, Luedeking-Piret equation and Luedeking-Piret/like equation. The kinetics model ofbiomass growth, β-glucan production, mannan synthesis and total sugar consumption were established, respectively. Through the calculation on Luedeking-Piret model, the results showed that the synthesis of β-glucan and mannan was link-coupled with cell. The experimental data were compared with the predicted ones, it showed the average relative error was generally less than 10%, which indicated that the kinetic models can reflect the dynamic characteristics of composite yeast fermentation process. The results showed that the models can guide the practical production significantly.
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