芝麻素的超声辅助提取技术及生物活性研究  

Ultrasound-assisted extraction and bioactivity of sesamin

作　　者：何晓梅[1,2] 陈存武[1,2] 乔德亮[1] 袁盼盼[1] 

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]植物细胞工程安徽省工程技术研究中心,安徽六安237012

出　　处：《中国油脂》2015年第5期65-69,共5页China Oils and Fats

基　　金：国家自然科学基金项目(31271853);安徽省自然科学基金(KJ2011A273;KJ2013B341);六安市定向委托项目(2010LWO12);皖西学院党建创新活动项目(WXXYDJ12003);皖西学院"生物质炼制"科技创新平台项目(44013010)

摘　　要：运用超声辅助90%乙醇溶液提取芝麻素。在单因素实验的基础上,运用正交实验优化芝麻素超声辅助提取工艺参数。普鲁士蓝法测定芝麻素还原能力,滤纸片-抑菌圈法测定其抗菌活性。结果表明:超声辅助提取的最优条件为超声功率300 W、液固比25∶1、超声温度55℃、超声时间25min,在最优条件下,芝麻素提取率为(3.942±0.085)mg/g;芝麻素具有一定的还原能力,但比相同质量浓度的VC弱得多;当质量浓度大于40 mg/m L时,芝麻素对蜡状芽孢杆菌、金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌均有不同程度的抑制作用,质量浓度大于60 mg/m L时其对4种细菌抑制作用的强弱顺序为蜡状芽孢杆菌>金黄色葡萄球菌>枯草芽孢杆菌>大肠杆菌,而当质量浓度小于100 mg/m L时,芝麻素对真菌黄曲霉无抑制作用。Sesamin was extracted by ultrasound-assisted 90% ethanol solution. On the basis of single factor experiment,the parameters of the ultrasound-assisted extraction of sesamin were optimized by orthogonal experiment. The reducing activity and the antibacterial activity of sesamin were determined by Prussian blue method and filter paper-inhibition zone method,respectively. The results showed that the optimal ultrasound-assisted extraction conditions were obtained as follows： ultrasonic power 300 W,ratio of liquid to solid 25∶ 1,ultrasonic temperature 55 ℃ and ultrasonic time 25 min. Under the optimal conditions,the extraction rate of sesamin was（ 3. 942 ± 0. 085） mg / g. Sesamin had certain reducing activity,much weaker than VCof the same mass concentration. When the mass concentration of sesamin was higher than 40 mg / mL,sesamin showed different degrees of inhibitory action on Bacillus cereus,Staphylococcus aureus,Bacillus subtilis and Escherichia coli,and the inhibitory action of sesamin with mass concentration higher than 60 mg / mL on Bacillus cereus was the strongest,followed by Staphylococcus aureus,Bacillus subtilis and Escherichia coli. When the mass concentration of sesamin was less than 100 mg / mL,no obvious inhibitory action of sesamin on Aspergillus flavus was observed.

关 键 词：芝麻 芝麻素 超声辅助提取 还原能力 抗菌活性 

分 类 号：TQ914.1[化学工程]