聚乙二醇/硫酸铵双水相体系萃取大豆脂肪氧合酶的研究  

Extraction of soybean lipoxygenase by PEG /(NH_4)_2SO_4 aqueous Two- phase systems

作　　者：丁文武[1] 丛林娜 孟丽 夏云空[4] 

机构地区：[1]西华大学,生物工程学院,四川成都610039 [2]中国科学院上海高等研究院,低碳转化科学与工程重点实验室,上海201210 [3]山东胜通集团股份有限公司,山东东营257500 [4]四川大学,生命科学学院,四川成都610065

出　　处：《食品工业科技》2015年第11期210-213,共4页Science and Technology of Food Industry

基　　金：西华大学2014年校重点科研基金项目(z1420523);四川省教育厅一般项目(15ZB0126)

摘　　要：采用聚乙二醇(PEG)/硫酸铵[(NH4)2SO4]双水相体系对大豆中脂肪氧合酶进行分离萃取研究;通过综合考察酶分配系数、相比和回收率,探讨了(NH4)2SO4、PEG2000、Na Cl浓度以及p H对脂肪氧合酶萃取的影响,并通过正交实验进一步优化实验条件,结果表明在单一因素下的最佳工艺条件为:(NH4)2SO415%(wt%)、PEG2000 13%(wt%)、Na Cl 1%(wt%)、p H5.8;正交实验优选出的最佳工艺条件为:(NH4)2SO417%(wt%)、PEG2000 13%(wt%)、p H5.2,可获得酶的分配系数最高可达9.710,萃取率为87.6%。Separation of lipoxygenase directly from nonfat soybean in aqueous two-phase systems (ATPS)of polyethylene glycoVammonium sulfate was studied. The effects of polyethylene glycol 2000 (PEG2000) concentration, (NH4)2SO4 concentration, NaCI concentration, and pH value on the extraction of soybean lipoxygenase were investigated in terms of partition coefficients, phase volume of both enzyme activity and recovery.Orthogonal experiment was used to further analyze and optimize the separation of lipoxygenase. The results indicated that the optimum separation conditions of single factor conditions were PEG2000 13%, (NH4) 2SO4 15%, NaCl1% and pH5.8. Maximum distribution coefficient and yield of lipoxygenase was 9.710 and 87.6% under the optimum separation conditions of PEG2000 13% , (NH4)2SO417% and pH5.2 obtained by orthogonal experiment.

关 键 词：脂肪氧合酶 双水相萃取系统 聚乙二醇 硫酸铵 

分 类 号：TS201.1[轻工技术与工程—食品科学]